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目的 :观察大鼠肠缺血再灌注后肺泡巨噬细胞活化分泌 NO及肺组织内 NOS的变化。方法 :建立实验大鼠肠缺血再灌注模型 ,采用 Griess法和分光光度法分别测定肺组织内 NO及NOS的活性。结果 :肠缺血再灌注组肺泡巨噬细胞分泌 NO的水平及肺内 NOS的水平均显著高于假手术对照组。结论 :肠缺血再灌注后肺内巨噬细胞的 i NOS被激活 ,大量合成并释放 NO,其作用一方面可增强杀菌功能 ,另一方面导致肺组织损伤
Objective: To observe the changes of NO production and NOS in alveolar macrophages after intestinal ischemia / reperfusion in rats. Methods: The model of intestinal ischemia / reperfusion in rats was established. The activity of NO and NOS in lung tissue were determined by Griess method and spectrophotometry respectively. Results: The level of NO secreted by alveolar macrophages and the level of NOS in lungs of intestinal ischemia-reperfusion group were significantly higher than those of sham operation control group. CONCLUSION: iNOS of macrophages in the lung is activated after intestinal ischemia-reperfusion, synthesizing and releasing NO in a large amount. Its function can enhance the bactericidal function on the one hand, and cause lung tissue damage on the other hand