The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb.The animals were allocated to two groups,and were administered chlorogenic acid or extract of S.lyratum Thunb.at a dose of 50.0 mg/kg orally.Blood samples were collected up to 8 h post-dosing.Plasma chlorogenic acid analyses were performed using an HPLC method with UV detector.The pharmacokinetic parameters were evaluated using non-compartmental assessment.Significant differences existed in the two groups for AUC 0 t,AUC 0 N and CLz/F.The reliable HPLC method was successfully applied to the determination of chlorogenic acid in rat plasma at dosage of 50.0 mg/kg. The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlorogenic acid or extract of S. lyratum Thunb. At a dose of 50.0 mg / kg orally. Blood samples were collected up to 8 h post-dosing. Plasma chlorogenic acid analyzes were performed using an HPLC method with UV detector. pharmacokinetic parameters were evaluated using non-compartmental assessment. Identification differences existed in two groups for AUC 0 t, AUC0N and CLz / F.The reliable HPLC method was successfully applied to the determination of chlorogenic acid in rat plasma at dosage of 50.0 mg / kg.