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通过活细胞计数法、 ＭＴＴ法、集落形成试验，研究了白头翁水提液（ＰＷＥ）和醇提液（ＰＡＥ）的体外抗肿瘤作用。结果：活细胞计数表明 ＰＷＥ对 ７７２１、Ｈｅｌａ、ＭＫＮ－４５细胞株 ７２ ｈ ＩＣ_ (５０)分别为 ０．８８、０．２８、０．８６ ｍｇ／ｍＬ，ＰＡＥ ５ ｍｇ／ｍＬ对 ３种细胞 ４８ ｈ生长抑制率分别为 ７８．７％、８９．５％、５８． ９％；ＰＷＥ对３种细胞降解ＭＴＴ ＩＣ_(５０)值分别为０．８２、０．６０、０．８１ｍｇ／ｍＬ，ＰＡＥ ５ ｍｇ／ｍＬ对 ３种细胞降解 ＭＴＴ抑制率分别为 ９０． ３％、９５． ６％、６２．１％；ＰＷＥ ２ ｍｇ／ｍＬ对 ３种细胞集落抑制率分别为 ４７． ８％、 ６３． ４％、 ４３． ６％； ＰＡＥ ５ ｍｇ／ｍＬ对 ３种细胞集落抑制率分别为 ６１． ７％、５４．６％、４５．３％。提示 ＰＷＥ、ＰＡＥ有直接细胞毒作用，并和时间、剂量呈正相关，且 ＰＷＥ作用比ＰＡＥ强。
The in vitro anti-tumor effects of P. cinnamomea extract (PWE) and alcohol extract (PAE) were studied by means of live cell counting, MTT assay and colony formation assay. RESULTS: The viable cell counts showed that the IC50 of PWE to 7721, HeLa, and MKN-45 cell lines was 0.88, 0.28, and 0.86 mg/mL respectively for 72 h, and PAE 5 mg/mL for 3 cells. h The growth inhibition rates were 78.7%, 89.5%, and 58. 9%; MTT IC_(50) values of PWE on three cell types were 0.82, 0.60, and 0.81 mg/mL, respectively, and PAE 5 mg/mL had 30% MTT inhibition rate on three cell types. 3%, 95. 6%, 62.1%; PWE 2 mg/mL inhibited the colony of the three kinds of cells were 47. 8%, 63. 4%, 43. 6%; PAE 5 mg/mL inhibited the colony of the three kinds of cells were 61. 7%, 54.6%, 45.3%. It is suggested that PWE and PAE have direct cytotoxicity and are positively correlated with time and dose, and PWE is more potent than PAE.