目的:通过对盆炎灵进行血清药物化学研究,初步阐明该复方的药效物质基础。方法:通过SD大鼠灌胃给予盆炎灵颗粒后,采用HPLC-DAD测定其含药血样、肝脏、肾脏、子宫的色谱图,通过与空白样品的HPLC指纹图谱对比分析,探讨盆炎灵的体内移行成分。结果:盆炎灵血清HPLC色谱图中可检出19个血中移行成分,其中11个成分被机体直接吸收入血,其余8个可能为代谢产物,5个色谱峰被确认为原儿茶醛、绿原酸、阿魏酸、甘草苷和丹酚酸B。含药肝脏组织中发现7个成分;含药肾脏中发现8个成分;含药子宫中发现2个成分。结论:含药组织的色谱峰明显多于空白组织,该方法为阐明盆炎灵的药效物质基础提供科学依据。 OBJECTIVE: To elucidate the pharmacological basis of this compound by studying the serum drug chemistry of Penyanling. Methods: After oral administration of Penyanling granule by intragastric administration in SD rats, the HPLC-DAD method was used to determine the chromatograms of the drug-containing blood, liver, kidney and uterus. By comparing with the HPLC fingerprints of blank samples, In vivo migration components. Results: Nineteen blood samples were detected by HPLC in pot Yanling serum. 11 of them were directly absorbed into the bloodstream by the body and the remaining 8 were metabolites. The five peaks were identified as protocatechuic aldehyde , Chlorogenic acid, ferulic acid, glycyrrhizin and salvianolic acid B. Seven components were found in medicated liver tissues; eight components were found in medicated kidneys; two components were found in medicated uterus. Conclusion: The chromatographic peaks of the drug-containing tissue are obviously more than that of the blank tissue. This method provides a scientific basis for elucidating the pharmacological basis of Penyanling.