目的:用高效液相色谱-电喷雾电离-质谱(HPLC-ESI-MS)法对银杏叶中银杏萜内酯进行分析。方法:利用LC-ESI-MS法对银杏叶样品进行了初步研究。液相色谱条件为色谱柱:Inertsil ODS 3,流动相:甲醇-水(36:64),流速1mL·min~(-);质谱为ZMD Micromass电喷雾质谱仪。结果:得到了样品总离子流和选择离子流图,及相应色谱峰的ESI-MS质谱图,对其进行了解析,鉴别出白果内酯及银杏内酯A、B、C、J。结论:本方法快捷、简单和准确。 Objective: To analyze the ginkgolide in ginkgo biloba leaves by high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Methods: A preliminary study of Ginkgo biloba leaf samples was conducted by LC-ESI-MS method. The HPLC conditions were column: Inertsil ODS 3, mobile phase: methanol-water (36:64), flow rate 1 mL·min~(-), mass spectrometry was ZMD Micromass electrospray mass spectrometer. RESULTS: The total ion flow and selected ion chromatograms of the samples were obtained, and the corresponding chromatographic peaks were analyzed by ESI-MS mass spectrometry. The results were analyzed and the ginkgolides and ginkgo lactones A, B, C, and J were identified. Conclusion: This method is quick, simple, and accurate.