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目的 探讨免疫健全的成年动物能否诱导供者同种异体心脏移植物的特异性免疫耐受。方法 以成年BN和DA雄性大鼠为供鼠，成年Lewis雄性大鼠为受鼠，给供鼠在切除脾脏或摘取心脏前腹腔注射地塞米松（Dex）。受鼠在手术前两周输注经Dex处理的供鼠脾脏细胞细胞（Dex-SpC）或者移植经Dex体内处理的供鼠心脏（Dex-Gr），或者联合进行上述两种处理（Dex-SpC+Dex-Gr）。另一组受鼠在输注Dex-SpC的前后3 d和围手术期间也用Dex进行了处理(Dex-SpC+Dex-Gr+Reci-Dex)。结果Dex-SpC组供者特异性移植物存活时间延长，而Dex-SpC+Dex-Gr组移植物存活时间更长。但是，Dex-SpC+Dex-Gr+Reci-Dex组移植物存活时间没有两联处理组长。结论 本研究证实了经Dex体内处理的供者脾细胞能够诱导师未经免疫抑制的成年动物的同种异体免疫耐受，提示耐受或排斥并非决定于抗原性质的“自我非我”，动物是新生还是成年。其机制可能是经Dex处理后脾细胞中的树突状细胞数量减少或功能降低，从而不能提供共刺激信号。
Objective To investigate whether immune-intact adult animals can induce specific immune tolerance of donor allograft heart grafts. Methods Male BN and DA rats were used as donors and adult Lewis male rats as recipients. Dexamethasone (Dex) was injected intraperitoneally into the anterior abdominal cavity of rats. The rats were infused with Dex-treated donor spleen cells (Dex-SpC) or Dex-Gr treated with Dex in vivo two weeks prior to surgery or both (Dex-SpC + Dex-Gr). The other group of mice were also treated with Dex (Dex-SpC + Dex-Gr + Reci-Dex) 3 d before and after infusion of Dex-SpC and during perioperative period. Results Dex-SpC donor-specific graft survival prolonged, while Dex-SpC + Dex-Gr graft survival longer. However, there was no double-team leader for Dex-SpC + Dex-Gr + Reci-Dex graft survival. Conclusions This study demonstrates that donor splenocytes treated with Dex in vivo are able to induce alloimmunity tolerance in adult immature immunocompromised adults, suggesting that tolerance or rejection does not depend on the nature of the “self-not” Whether the animal is new or adult. The mechanism may be Dex-treated spleen cells in the number of dendritic cells decreased or decreased function, which can not provide costimulatory signals.