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以抗、感疫霉根腐病的2个品种和一对矮秆突变体与野生型为材料,研究BR对抗大豆疫霉根腐病防御反应的过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)活性的影响。目的是明确BR对抗大豆疫霉根腐病的作用效果,鉴定矮秆突变体的抗性。酶活测定表明,接种使大豆叶片的POD和PAL活性显著增强,说明病原物诱导使植株产生系统抗病性;加BR接种处理(B~+P~+)比加BR不接种处理(B~+P~-)的POD和PAL活性有一定的增强,表明BR对抗大豆疫霉根腐病有一定的抗性效果;挑战接种鉴定表明,东农42属于感病,而东泽11属于中间类型,施加BR后对这两个品种的抗性均有增强作用。
Two varieties of resistant and susceptible root rot, one pair of dwarf mutant and wild type were used to study the effects of BR on peroxidase (POD) and phenylalanine Aminotransferase (PAL) activity. The purpose is to clarify the effect of BR against Phytophthora sojae and to identify the resistance of dwarf mutants. Enzyme activity assay showed that POD and PAL activities in soybean leaves were significantly increased after inoculation, indicating that the pathogen induced systemic resistance in plants. Inoculation with BR (B ~ + P ~ +) + P ~ -) showed a certain increase in POD and PAL activity, indicating that BR has certain resistance to the attack of Phytophthora sojae; Challenge inoculation identification showed that Dongnong 42 was susceptible, while Dongze 11 belonged to the intermediate type , The resistance of these two cultivars enhanced after BR was applied.