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目的观察高功率微波辐射(high power microwave radiation,HPM)对小鼠心肌氧化应激及ATPase活性的影响。方法雄性ICR小鼠32只,随机分为4组(n=8):假照组、微波辐射10 min组、微波辐射20 min组、微波辐射30 min组。采用S波段,平均表面功率为10 mW/cm2微波;照射时间分别为0,10,20,30 min/次,每周2次,共2周。末次辐射完毕,立即处死小鼠,取心脏、冰冷生理盐水冲洗后,加入匀浆液制作匀浆。采用试剂盒测定超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-px)活性、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)及丙二醛(MDA)的含量以及Na+K+-ATPase和Ca2+Mg2+-AT-Pase活性。结果微波辐射后,与假照组比,微波辐射20 min组和微波辐射30 min组SOD活性降低(P<0.05)、GSH-px活性增加(P<0.05);而微波辐射20 min组GSH含量明显增加(P<0.01);微波辐射30 min组Ca2+Mg2+-ATPase活性明显降低(P<0.01),其他指标变化无明显统计学差异。结论微波辐射可能引起小鼠心脏氧化应激平衡状态以及Ca2+转运的改变。
Objective To observe the effects of high power microwave radiation (HPM) on myocardial oxidative stress and ATPase activity in mice. Methods Thirty-two male ICR mice were randomly divided into 4 groups (n = 8): sham group, 10 min of microwave irradiation, 20 min of microwave irradiation and 30 min of microwave irradiation. Using S band, the average surface power of 10 mW / cm2 microwave; irradiation time were 0,10,20,30 min / times, twice a week for 2 weeks. The last radiation is completed, the mice were sacrificed immediately, take the heart, washed with cold saline, adding homogenate homogenate. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-px), total antioxidant capacity (T-AOC), glutathione (GSH) and malondialdehyde (MDA) content and Na + K + -ATPase and Ca2 + Mg2 + -AT-Pase activity. Results Compared with sham irradiation group, the activity of GSH-px increased (P <0.05) and the activity of GSH-px increased (P <0.05) in microwave irradiation for 20 min and microwave irradiation for 30 min, (P <0.01). The activity of Ca2 + Mg2 + -ATPase in the group treated with microwave for 30 min was significantly lower than that in the control group (P <0.01). There was no significant difference in other indexes. Conclusion The microwave radiation may cause the heart oxidative stress balance state and Ca2 + transport changes in mice.