论文部分内容阅读
目的:观察早期肠内谷氨酰胺补给对烫伤大鼠回肠p-ERK1/2表达的影响．方法:选健康SD大鼠88只随机分为肠内营养组(EN组,n=40)、肠内营养加谷氨酰胺补给组(EN+Gln组,n=40)和伤前对照组(n=8),制备总烧伤面积(TBSA)为30%的Ⅲ~0烫伤动物模型,伤后早期分别给予肠内营养制剂能全力和能全力加Gln,于6,12,24,48和96 h观察肠黏膜细胞p-ERK的表达分布及血浆D-乳酸浓度变化．结果:烧伤后血浆D-乳酸水平升高,EN组在观察时间内血浆D-乳酸水平没有恢复正常(P＜0．05),EN+Gln组血浆D-乳酸水平在伤后48 h恢复至伤前水平且明显低于EN组(4．7±0．9 mmol/L vs 6．9±1．2 mmol/L,P＜0．05)．p-ERK1/2免疫组化染色显示烧伤后阳性细胞数量显著增加,EN+Gln组回肠阳性细胞百分比在24,48,96 h明显高于EN组(绒毛:87．6%±9．6%．84．4%±10．3%,74．6%±9．7% vs 64．6%±7．3%,59．6%±7．1%,58．4%±7．4%,P＜0．05;隐窝:73．6%±11．2%,67．4%±8．6%,63．6%±7．9% vs 54．3%±6．3%,51．6%±5．9%,48．4%±5．3%,P＜0．05)．Western blot显示EN组伤后6 h p-ERK1/2表达明显增加,12 h达高峰,然后逐渐下降,EN+Gln在24 h达到高峰并逐渐下降．结论:相对于肠内营养,早期肠内给予Gln能促进肠上皮细胞p-ERK1/2的表达,改善黏膜局部的屏障功能．
Objective: To observe the effects of early enteral glutamine supplementation on ileal p-ERK1 / 2 expression in scalded rats. Methods: Eighty healthy SD rats were randomly divided into three groups: enteral nutrition group (EN group, n = 40), enteral nutrition plus glutamine supplementation group (EN + Gln group, n = 40) and pre-injury control group n = 8). The scalded animals of Ⅲ ~ 0 scald with a total burn area (TBSA) of 30% were prepared. The enteral nutrition preparations were given full and full dose of Gln at the early stage of injury, respectively. At 6,12,24,48 and 96 h observed the expression of p-ERK in intestinal mucosa cells and plasma concentration of D-lactic acid. Results: The level of plasma D-lactate increased after burn, the level of plasma D-lactate did not return to normal in the EN group (P <0.05), and the level of D-lactate in EN + Gln group recovered to 48 h after injury Pre-injury levels were significantly lower than those in the EN group (4.7 ± 0.9 mmol / L vs 6.9 ± 1.2 mmol / L, P <0.05). Immunohistochemical staining of p-ERK1 / 2 showed a significant increase in the number of positive cells after burn. The percentage of ileal positive cells in EN + Gln group was significantly higher than that in EN group at 24,48 and 96 h (villus: 87.6% ± 9.6% .84.4% ± 10.3%, 74.6% ± 9.7% vs 64.6% ± 7.3%, 59.6% ± 7.1%, 58.4% ± 7.4% , P <0.05; crypts: 73.6% ± 11.2%, 67.4% ± 8.6%, 63.6% ± 7.9% vs 54.3% ± 6.3% 51.6% ± 5.9%, 48.4% ± 5.3%, P <0.05). Western blot showed that the expression of p-ERK1 / 2 increased significantly at 6 h after injury in EN group, peaked at 12 h, then decreased gradually, and peaked at 24 h after EN + Gln decreased gradually. CONCLUSIONS: Compared with enteral nutrition, early enteral administration of Gin can promote p-ERK1 / 2 expression in intestinal epithelial cells and improve mucosal barrier function.