论文部分内容阅读
目的 :研究大肠恶性肿瘤的转移相关基因血管内皮细胞生长因子 (VEGF)和CD4 4粘附分子的表达及其临床意义。方法 :应用逆转录 聚合酶链反应 (RT PCR)方法检测 1997年 12月— 1998年 5月间收治的 2 1例大肠恶性肿瘤。其中 19例为大肠癌 ,1例为平滑肌肉瘤 ,1例为转移性腺样囊腺癌。大肠癌Dukes分期为B期6例 ,C期 9例及D期 4例。上述标本采用RT PCR方法检测各类基因的mRNA表达。结果 :大肠恶性肿瘤VEGF阳性表达 10例 ,另 11例为阴性结果。而CD4 4阳性表达 8例 ,另 13例为阴性。大肠癌的不同分期与VEGF和CD4 4表达稍有不同 ,但两者随肿瘤分期的进展而呈现阳性结果增高。结论 :转移基因的VEGF和CD4 4表达 ,在大肠癌的生物学及临床分期研究中可作为判别指标
Objective: To study the expression of vascular endothelial growth factor (VEGF) and CD4 (superscript 4 +) adhesion molecule in metastatic colorectal cancer and its clinical significance. Methods: Twenty-one cases of colorectal malignant tumors admitted from December 1997 to May 1998 were detected by reverse transcription-polymerase chain reaction (RT-PCR). Of these, 19 were colorectal cancer, 1 was leiomyosarcoma, and 1 was metastatic adenoid cystadenocarcinoma. Colorectal cancer Dukes stage B in 6 cases, C in 9 cases and D in 4 cases. The above specimens using RT PCR method to detect various types of gene mRNA expression. Results: The positive expression of VEGF in 10 cases of colorectal malignant tumors, the other 11 cases were negative results. CD4 4 positive expression in 8 cases, the other 13 cases were negative. The different stages of colorectal cancer with VEGF and CD4 4 expression slightly different, but both with the progress of the tumor staging showed positive results increased. Conclusion: The expression of VEGF and CD4 4 in metastatic genes may be used as a discriminant in the biological and clinical staging of colorectal cancer