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目的:采用高效液相色谱法建立安络小皮伞提取物的特征图谱。方法:通过对安络小皮伞提取物1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化,采用高效液相色谱法进行单糖组成分析。结果:安络小皮伞提取物特征图谱共检出5个峰,其中已通过对照品确定5个色谱峰的归属,安络小皮伞提取物水解产物中含有甘露糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖。结论:该方法简单,快速,灵敏度高,重现性良好,可作为控制安络小皮伞提取物内在质量的标准。
OBJECTIVE: To establish a characteristic map of the extract of Pinus edulis with high performance liquid chromatography. Methods: Mono-saccharide composition analysis was carried out by precolumn derivatization of 1-phenyl-3-methyl-5-pyrazolone (PMP) Results: Five peaks were detected in the characteristic map of the extract of Anlao brachilu, of which five peaks were identified by the reference substance. The hydrolyzate of the extract of Anlao brachilu spp. Contained mannose, galacturonic acid, Glucose, galactose, arabinose. Conclusion: The method is simple, rapid, sensitive and reproducible. It can be used as a standard to control the intrinsic quality of the extract.