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目的:研究富亮氨酸胶质瘤失活基因1(LGI1)与胶质瘤细胞生长及胶质瘤细胞凋亡的关系。方法:构建pcDNA3.1/LGI1质粒,采用脂质体介导的方法转染LGI1缺失的胶质瘤细胞系。RT-PCR法和免疫组化法检测转染细胞的LGI1基因表达,MTT法检测转染细胞的增殖活性,AnnevinV-FITC法检测转染细胞的凋亡。结果:pcDNA3.1/LGI1转染胶质瘤细胞系A172后,A172细胞LGI1mRNA和蛋白表达阳性;细胞增殖活性受到抑制,MTT吸收值在转染后24和48小时下降,与正常对照组相比有统计学意义(P<0.05);转染后24小时早期凋亡细胞较多,48小时后早期凋亡减少。结论:LGI1基因可抑制肿瘤细胞的增殖,诱导细胞凋亡。
Objective: To investigate the relationship between leuco-leukemic inactivation gene 1 (LGI1) and glioma cell growth and glioma cell apoptosis. Methods: pcDNA3.1 / LGI1 plasmid was constructed and transfected into LGI1-deficient glioma cell line by liposome-mediated method. The expression of LGI1 gene was detected by RT-PCR and immunohistochemistry. The proliferation of transfected cells was detected by MTT assay. The apoptosis of transfected cells was detected by AnnevinV-FITC. Results: After transfection with pcDNA3.1 / LGI1, the expression of LGI1 mRNA and protein in A172 cells was inhibited after transfected with glioma cell line A172. The cell proliferation activity was inhibited and the MTT absorption decreased at 24 and 48 hours after transfection compared with the normal control group (P <0.05). There were more apoptotic cells in the early 24 hours after transfection and less apoptosis in the early 48 hours after transfection. Conclusion: The LGI1 gene can inhibit the proliferation of tumor cells and induce apoptosis.