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目的:湿磨法制备山药多糖纳米粒,星点设计优化最优处方。研究山药多糖纳米粒的体外抗氧化活性。方法:通过星点设计优化湿磨法制备的山药多糖纳米粒最优处方,SALD-2201激光粒度分布仪检测其平均粒径及粒度分布。通过化学实验法和试剂盒法研究过氧化氢、羟自由基、超氧阴离子自由基、DPPH的抗氧化能力,并测定其还原力与金属螯合力。结果:星点设计得最优工艺料液比1∶24、球磨转速360 r·mim-1、球磨时间36 h。SALD-2201激光粒度分布仪测定1.0%粒径在186 nm,80.0%粒径575 nm。经体外抗氧化实验证实,过氧化氢、DPPH、还原潜力与金属螯合力的自由基清除率随多糖浓度的增加而成增长趋势。其中山药多糖与其纳米粒对DPPH清除能力在0.5,4,6,8 g·L-1质量浓度下均有极显著差异。超氧阴离子自由基与羟自由基的抗氧化能力亦与多糖浓度成正相关。结论:山药多糖及其纳米粒体外抗氧化活性良好,且山药多糖纳米粒的体外抗氧化活性优于山药多糖。
OBJECTIVE: To prepare yam polysaccharide nanoparticles by wet milling and optimize the prescription of star points. In vitro anti-oxidative activity of yam polysaccharide nanoparticles. Methods: Optimal formulation of yam polysaccharide nanoparticles prepared by wet grinding method was optimized by star point design. The average particle size and particle size distribution of yam polysaccharide nanoparticles were determined by SALD-2201 laser particle size distribution analyzer. The antioxidant activity of hydrogen peroxide, hydroxyl radical, superoxide anion radical and DPPH were studied by chemical experiment and kit method. The reducing power and metal chelating ability were also determined. Results: The optimal ratio of star to material was 1:24, the milling speed was 360 r · mim-1 and the milling time was 36 h. SALD-2201 laser particle size distribution analyzer measured 1.0% particle size at 186 nm, 80.0% particle size 575 nm. Antioxidant experiments in vitro confirmed that hydrogen peroxide, DPPH, reduction potential and metal chelating ability of free radical scavenging rate with the increase of polysaccharide concentration and the growth trend. Among them, the yam polysaccharides and their nanoparticles showed significant difference in DPPH scavenging ability under the concentrations of 0.5, 4, 6 and 8 g · L-1. The antioxidant capacity of superoxide anion radical and hydroxyl radical also positively correlated with polysaccharide concentration. Conclusion: The antitumor activity of polysaccharide and its nanoparticles in vitro is good, and the antioxidant activity of yam polysaccharide nanoparticles in vitro is better than that of yam polysaccharide.