本文采用免疫组织化学方法对成年大鼠海马巢蛋白阳性神经元及其出生后的发育变化进行了研究。结果显示 ,在成年大鼠海马 CA1、CA2和 CA3区锥体层某些特定部位的神经元呈巢蛋白阳性反应。巢蛋白阳性神经元出生后 3周开始在海马中出现 ,并且到 12月龄大鼠海马内仍有存在。双重反应结果显示 ,巢蛋白与 GABA在海马神经元内无共存。成年大鼠海马锥体层神经元表达巢蛋白的生物学意义尚不清楚 In this paper, immunohistochemistry was used to detect the expression of nestin positive neurons in adult hippocampus and its development after birth. The results showed that the neurons in some specific parts of hippocampal CA1, CA2 and CA3 pyramidal layer of rat hippocampus were positive for nestin. Nestin positive neurons begin to appear in the hippocampus three weeks after birth and still remain in the hippocampus of 12 month old rats. Double reaction results showed that nestin and GABA did not coexist in hippocampal neurons. The biological significance of nestin expression in pyramidal neurons of adult rat hippocampus is not clear