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土壤甲烷代谢微生物影响甲烷的产生和氧化,然而关于林型对土壤中甲烷代谢微生物群落结构影响的研究较少。采用基因芯片GeoChip 3.0研究了湿地松人工林和马尾松人工林土壤甲烷代谢微生物群落结构特征。结果如下,(1)两种林型的甲烷代谢微生物群落结构存在极显著差异(P=0.008),林型能解释其34.9%的变异;(2)产甲烷菌(包含甲基辅酶M还原α亚基基因mcrA的微生物)的优势菌群发生了变化,湿地松人工林的的优势菌为Methanocorpusculum labreanum Z,马尾松人工林的优势菌群除Methanocorpusculum labreanum Z外,还包括产甲烷古菌和Methanosarcina mazei Gol;(3)甲烷营养菌(包含甲烷单加氧酶基因pmoA基因的微生物)的优势菌为Ⅱ型,有3种不可培养细菌只在湿地松人工林检测到,在马尾松人工林中未检测到;(4)mcrA基因丰度或同源基因数量与土壤容重正相关,与土壤粘粒含量呈显著负相关;pmoA基因信号强度或多样性指数与土壤全碳含量、全磷含量和速效氮含量显著正相关。总之,相比本地种马尾松人工林,引进种湿地松人工林的土壤甲烷代谢微生物群落结构发生了显著变化。
Soil methane metabolism microorganisms affect methane production and oxidation, however, there are few studies on the impact of forest types on the community structure of methane metabolism microorganisms in soil. GeoChip 3.0 was used to study the structural characteristics of soil methane metabolism microbial community in Pinus elliottii plantations and Pinus massoniana plantations. The results are as follows: (1) There was a significant difference (P = 0.008) in the community structure of methane metabolism among the two forest types, and 34.9% variation was explained by the forest type. (2) Methanogenic bacteria Subunit gene mcrA microorganisms) dominant flora changed, the dominant bacteria in the Pinus elliottii plantation Methanocorpusculum labreanum Z, Masson Pine plantation dominant flora in addition to Methanocorpusculum labreanum Z, but also include methanogenic archaea and Methanosarcina mazei Gol; (3) The dominant bacteria of methanotrophs (microorganisms containing pmoA gene of methane monooxygenase gene) were type Ⅱ, and three kinds of uncultured bacteria were detected only in Pinus elliottii plantations, (4) The mcrA gene abundance or the number of homologous genes positively correlated with soil bulk density, and negatively correlated with soil clay content. The signal intensity or diversity index of pmoA gene correlated with soil total carbon content, total phosphorus content and Available nitrogen content was significantly and positively correlated. In conclusion, compared with the native Pinus massoniana plantations, the structure of methane metabolism microbial community in the introduced plantation of Pinus elliottii significantly changed.