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目的:探讨淫羊藿总黄酮(TFE)对喂养高脂饮食的大鼠主动脉一氧化氮(NO)及一氧化氮合酶(NOS)的影响以及抗动脉粥样硬化(AS)的可能机制。方法:将35只SD大鼠随机分为普通饮食组(n=9)和高脂饮食组(n=26)。后者喂以高脂饮食12周后,检测空腹血脂,若已出现高脂血症说明造模成功,再随机分为模型组、小剂量[100 mg/(kg.只)]TFE组及大剂量[200 mg/(kg.只)]TFE组。16周后,检测各组血脂水平,三酰甘油(TG)用TPO-PAP法检测,总胆固醇(TC)用CHOD-PAP法检测,低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)用选择性沉淀法检测。主动脉NO水平通过硝酸还原法检测、用精氨酸催化法检测主动脉总NOS(tNOS)、诱导型NOS(iNOS)及构成型NOS(cNOS)(等于tNOS与iNOS之差)的水平。结果:以高脂饮食喂养12周后,高脂饮食组TG、TC及LDL的水平明显升高(P<0.01)。给药4周后,与模型组比较,TFE组大鼠的血脂谱能明显改善,TC和TG的水平明显降低(P<0.05,P<0.01)。模型组主动脉NO和cNOS的水平显著下降(P<0.05,P<0.01),TFE组主动脉NO和cNOS的水平显著提高(P<0.05,P<0.01)。结论:TFE能改善血脂及提高主动脉NO、cNOS的水平。
Objective: To investigate the effect of epimedium saponin (TFE) on aortic nitric oxide (NO) and nitric oxide synthase (NOS) in rats fed a high-fat diet and its possible mechanism against atherosclerosis (AS) . Methods: 35 SD rats were randomly divided into normal diet group (n = 9) and high fat diet group (n = 26). The latter fed with a high-fat diet for 12 weeks, fasting blood lipids, if hyperlipidemia has been successful, and then randomly divided into model group, low-dose [100 mg / (kg. Dose [200 mg / (kg. Only)] TFE group. Twenty-six weeks later, the levels of lipids in each group were measured, the triglyceride (TG) was detected by TPO-PAP method, the total cholesterol (TC) by CHOD-PAP method, the levels of low density lipoprotein cholesterol (LDL-C) and high density lipoprotein Cholesterol (HDL-C) was detected by selective precipitation. Aortic NO levels were measured by nitric acid reduction. The levels of aorta total NOS (tNOS), inducible NOS (iNOS) and constitutive NOS (cNOS) (equal to the difference between tNOS and iNOS) were measured by arginine catalytic assay. Results: After fed with high-fat diet for 12 weeks, the levels of TG, TC and LDL in high-fat diet group were significantly increased (P <0.01). After 4 weeks of administration, compared with the model group, the blood lipid profile of TFE group was significantly improved, and the levels of TC and TG were significantly decreased (P <0.05, P <0.01). The level of NO and cNOS in the aorta decreased significantly in the model group (P <0.05, P <0.01), while the level of NO and cNOS in the aorta increased significantly in the TFE group (P <0.05, P <0.01). Conclusion: TFE can improve blood lipids and improve aortic NO, cNOS levels.