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以尼泊金乙酯(EP)为模板分子,邻苯二胺(o-PD)为功能单体,利用循环伏安法在玻碳电极(GCE)表面制备分子印迹膜,对分子印迹聚合条件进行了优化,并进一步在双通道丝网印刷碳电极(SPCE)表面进行电聚合,制得双通道尼泊金乙酯分子印迹膜电极(EP-MIP/SPCE)。利用循环伏安法、方波伏安法和电流-时间曲线法对分子印迹传感器的电化学性能进行评价。EP在双通道EP-MIP/SPCE上的氧化电流与EP在低浓度区(3.2×10~(-7) mol/L~3.2×10~(-6) mol/L)和高浓度区(2.7×10~(-5) mol/L~5.6×10~(-4) mol/L)分别呈良好的线性关系,检出限为9.7×10~(-8) mol/L。运用建立的方法对市售酱油中的EP进行了测定,加标回收率为97.2%~106.6%,实验结果表明,该双通道分子印迹传感器可对酱油等食品样品中的尼泊金酯类进行快速测定。
The molecular imprinted membrane was prepared on the surface of glassy carbon electrode (GCE) by cyclic voltammetry using ethylparaben (EP) as template and o-PD as functional monomer. The molecular imprinting conditions (EP-MIP / SPCE) was prepared by electropolymerization on the surface of double-pass screen-printed carbon electrode (SPCE). The electrochemical performance of molecular imprinting sensor was evaluated by cyclic voltammetry, square wave voltammetry and current-time curve method. The oxidation current of EP on EP-MIP / SPCE was similar to that of EP in low concentration (3.2 × 10 -7 mol / L ~ 3.2 × 10 -6 mol / L) and high concentration (2.7 × 10 -5 mol / L ~ 5.6 × 10 -4 mol / L, respectively. The detection limit was 9.7 × 10 ~ (-8) mol / L. The established method was applied to the determination of EP in commercial soy sauce, with the recoveries of 97.2% -106.6%. The experimental results show that the two-channel molecular imprinting sensor can detect nipagin esters in food samples such as soy sauce Rapid determination.