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目的 通过检测SMAD4mRNA、P73mRNA在肝外胆管癌中表达 ,研究它们在肝外胆管癌发生、发展过程中的表达特点及相互关系、作用机制及其临床价值。方法 应用石蜡包块切片组织原位杂交染色法检测 4 2例肝外胆管癌中SMAD4mRNA、P73mRNA的表达 ,并以同期 2 0例慢性胆管炎作对照。结果 ①SMAD4mRNA阳性表达率为 6 1.90 % (2 6 /42 ) ,显著低于其相应良性对照组 (为 10 0 % ) ;P73mRNA表达率为 5 4 .76 % (2 3/42 ) ,显著高于相应良性对照组 (为 0 % )。②SMAD4mRNA、P73mRNA的表达与其临床病理分期及预后显著相关 (P <0 .0 5 ) ;SMAD4mRNA的表达与组织学分级及是否发生周围神经、血管、淋巴管浸润转移显著相关 (P <0 .0 5 )。③SMAD4mRNA、P73mRNA的表达两者呈负相关。结论 SMAD4mRNA、P73mRNA在肝外胆管癌中的表达反映了肝外胆管癌生物学特性 ,为预后判断提供参考指标 ,并为探索肝外胆管癌早期诊断和干预性基因治疗提供实验依据。
Objective To detect the expression of SMAD4 mRNA and P73 mRNA in extrahepatic cholangiocarcinoma and to study the expression, correlation and mechanism of them and their clinical value in the occurrence and development of extrahepatic cholangiocarcinoma. Methods The expression of SMAD4 mRNA and P73 mRNA in 42 cases of extrahepatic cholangiocarcinoma was detected by paraffin embedded tissue section in situ hybridization staining and compared with 20 cases of chronic cholangitis in the same period. Results ① The positive rate of SMAD4 mRNA was 6 1.90% (26/42), which was significantly lower than that of the corresponding benign control group (10 0%). The P73 mRNA expression was 54.76% (2 3/42) The corresponding benign control group (0%). The expression of SMAD4mRNA and P73mRNA was significantly correlated with the clinicopathological stage and prognosis (P <0.05); The expression of SMAD4mRNA was significantly correlated with the grading of histology and infiltration and metastasis of peripheral nerves, blood vessels and lymphatic vessels (P <0.05) ). ③SMAD4mRNA, P73mRNA expression were negatively correlated. Conclusion The expression of SMAD4 mRNA and P73 mRNA in extrahepatic cholangiocarcinoma reflects the biological characteristics of extrahepatic cholangiocarcinoma and provides a reference index for the prognostic judgment. It also provides an experimental basis for exploring the early diagnosis and interventional gene therapy of extrahepatic cholangiocarcinoma.