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目的:探讨过氧化物酶体增殖物激活受体(peroxisome proliferater-activated receptors,PPAR)α的配体——非诺贝特、WY14643,PPARγ配体——匹格列酮对脂多糖(LPS)诱导的人单核细胞株-THP-1细胞表达组织因子(TF)的影响。方法:采用RT-PCR法检测单核细胞THP-1的TF mRNA表达水平,用免疫细胞化学法检测细胞TF蛋白表达量。结果:PPAR配体处理组中THP-1细胞TF mRNA水平以及蛋白表达量均较单纯LPS刺激组明显降低。结论:3种PPAR配体均可抑制单核细胞TF mRNA以及蛋白表达,可能经此机制减轻动脉粥样硬化病变的发生。
OBJECTIVE: To investigate the effect of ligand-fenofibrate, WY14643 and PPARγ ligand-pioglitazone on lipopolysaccharide (LPS) in peroxisome proliferator-activated receptors (PPAR) Induced human monocytic line-THP-1 cells to express tissue factor (TF). Methods: The mRNA expression level of THP-1 in monocytes was detected by RT-PCR. The expression of TF protein was detected by immunocytochemistry. Results: The levels of TF mRNA and the protein level of THP-1 cells in PPAR ligand-treated group were significantly lower than those in LPS alone group. CONCLUSION: All three PPAR ligands can inhibit the expression of TF mRNA and protein in monocytes. This mechanism may reduce the occurrence of atherosclerotic lesions.