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目的研究系统性红斑狼疮(SLE)患者外周血调节性T细胞(Treg)的变化及其与病情活动的相关性,探讨Treg细胞在SLE发病机制中的作用。方法用四色分选流式细胞仪检测103例SLE患者及58名健康对照人群外周血Treg细胞百分数(%)。SLE活动性的判断采用SLEDAI评分方法,其中非活动期(SLEDAI≤9分)患者37例,活动期(SLEDAI>9分)患者66例。分析以上SLE患者外周血Treg细胞百分数的变化及其与病情活动的相关性。结果①SLE患者组Treg细胞(%)(1.59±1.21)显著低于健康对照组(2.62±1.76),P<0.05;②SLE活动组Treg细胞(%)(1.53±1.28)显著低于SLE非活动组(2.14±1.97),P<0.05,SLE活动组Treg细胞(%)(1.53±1.28)显著低于健康对照组(2.62±1.76),P<0.05,SLE非活动组Treg细胞(%)(2.14±1.97)显著低于健康对照组(2.62±1.76),P<0.05。③Treg细胞百分数与SLEDAI评分呈显著负相关。结论 SLE患者外周血Treg细胞百分数存在显著异常,并与疾病活动性密切相关,说明Treg细胞可能在SLE的发生、发展中起重要作用,为SLE的发病机制和治疗提供新的思路。
Objective To investigate the changes of Tregs in peripheral blood and their correlation with disease activity in patients with systemic lupus erythematosus (SLE) and to explore the role of Tregs in the pathogenesis of SLE. Methods The percentages of Treg cells in peripheral blood of 103 SLE patients and 58 healthy controls were detected by four-color sorting flow cytometry. The SLEDAI score was used to determine the activity of SLE, including 37 inactive patients (SLEDAI≤9 points) and 66 active patients (SLEDAI> 9 points). Analysis of the above SLE patients with peripheral blood Treg cell percentage changes and their relationship with the disease activity. Results ① The percentage of Treg cells (1.59 ± 1.21) in SLE patients was significantly lower than that in healthy controls (2.62 ± 1.76), P <0.05. ② The percentage of Treg cells in patients with SLE was significantly lower than that in inactive SLE patients (1.53 ± 1.28) (2.14 ± 1.97), P <0.05, the percentage of Treg cells in SLE activity group (1.53 ± 1.28) was significantly lower than that in healthy control group (2.62 ± 1.76), P <0.05 ± 1.97) was significantly lower than the healthy control group (2.62 ± 1.76), P <0.05. ③Treg cell percentage and SLEDAI score was significantly negatively correlated. Conclusion The percentage of Treg cells in peripheral blood of patients with SLE is significantly abnormal and closely related to disease activity, indicating that Treg cells may play an important role in the pathogenesis and development of SLE and provide new ideas for the pathogenesis and treatment of SLE.