目的　探讨生殖支原体、人微小病毒B19感染与围产儿结局不良的关系。方法　应用聚合酶链反应技术 ,对 5 7例围产儿结局不良患者的静脉血、脐血、羊水、乳汁、胎盘 ,尸体的脑、肝、肾、脾、肺、骨髓 ,及 44例正常分娩产妇的静脉血、脐血、羊水、乳汁、胎盘进行生殖支原体 DNA、B19 DNA检测 ,了解其感染情况。结果　异常组中生殖支原体、B19病毒感染高于对照组 ,以胎盘组织中感染率最高。 19例死胎死产中 ,胎盘组织生殖支原体、B19病毒感染率达 6 8 42 % ( 13/ 19) ;2 9例畸形儿胎盘中感染率达 34 48% ( 10 / 2 9)。尸检儿脏器中B19病毒感染顺位为脾、肝、骨髓、脑、肺、肾 ;生殖支原体感染顺位为肝、肺、脑、脾、肾。结论　生殖支原体、B19病毒感染与围产儿结局不良关系密切 ,胎盘组织是其易感组织 Objective To investigate the relationship between mycoplasma genitalium, human parvovirus B19 infection and poor perinatal outcome. Methods Polymerase chain reaction (PCR) was performed in 52 cases of venous blood, umbilical cord blood, amniotic fluid, milk, placenta, cadaver’s brain, liver, kidney, spleen, lung and bone marrow of 57 poor perinatal children with end- Of venous blood, umbilical cord blood, amniotic fluid, milk, placenta Mycoplasma genitalium DNA, B19 DNA test to understand the infection. Results In the abnormal group, mycoplasma genitalium and B19 virus infection were higher than those in the control group, with the highest infection rate in placenta. Among 19 stillbirths, the infection rate of Mycoplasma genitalium and B19 was 6 8 42% (13/19). The infection rate in 29 fetuses with abnormality was 34 48% (10/29). The positive rate of infection of B19 virus in the autopsy organs was spleen, liver, bone marrow, brain, lung and kidney. The prevalence of mycoplasma genitalium was liver, lung, brain, spleen and kidney. Conclusions Mycoplasma genitalium and B19 infection are closely related to the poor outcome of perinatal infants. Placenta is the predominant tissue