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本实验表明,人rIL-4可以促进SAC活化的人扁桃腺B细胞的增殖,且预活化24小时和72小时的B细胞均对IL-4有相似程度的增殖,只是24小时活化的B细胞有较高的本底应答.然而rIL-4支持活化后B细胞增殖的时间相当短,在最初的24小时培育中,B细胞对IL-4有良好的增殖应答,培育在72小时时,B细胞的增殖应答则显著降低。同时表明,以SAC—B细胞测定rIL-4活性的适宜方法是二步法,第一步培育72小时,第二步与rIL-4共育24小时。不溶性Anti-μ活化的B细胞对SAC的增殖应答较SAC活化者为高。无论SAC或Anti-μ活化的B细胞对rIL-2和rIL-4均应答良好,故不能区分在含有此两种因子的天然样品中各因子的单独活性。
This experiment showed that human rIL-4 can promote the proliferation of SAC-activated human tonsil B cells, and the B cells pre-activated for 24 hours and 72 hours all have a similar degree of proliferation of IL-4, only 24 hours of activated B cells However, rIL-4 supports a relatively short time to proliferate B cells after activation, and B cells have a good proliferative response to IL-4 during the first 24 hours of incubation. After 72 hours incubation, B Cell proliferation response was significantly reduced. Simultaneously, it is shown that a suitable method for measuring the activity of rIL-4 by SAC-B cells is a two-step method, which is incubated for 72 hours in the first step and 24 hours for rIL-4 in the second step. Insulin-resistant activated B cells proliferate more in SAC than SAC activators. Whether SAC or Anti-μ activated B cells responded well to both rIL-2 and rIL-4, the individual activity of each factor in natural samples containing both of these factors could not be distinguished.