论文部分内容阅读
目的探讨增损甘露消毒丹抗肝纤维化的疗效及作用机理。方法采用四氯化碳皮下注射和酒精灌胃等复合因素建立大鼠肝纤维化模型,设6组,各组用药8周。治疗结束后采用放射免疫法检测大鼠血清III型前胶原(PCIII)、IV型胶原(IV-C)的含量。采用胶原纤维(Van-Gieson)染色法和网状纤维(Gomori)染色法光镜下观察并进行肝纤维化病理分级;网状纤维增生程度采用计算机图像分析系统进行分析。结果病理模型组大鼠血清PCIII、IV-C含量均较正常组明显升高(P<0.05);经用药治疗后,增损甘露消毒丹大剂量组、中剂量组、复方鳖甲软肝片组血清PCIII、IV-C含量与病理模型组比较明显降低(P<0.05);增损甘露消毒丹大剂量组、中剂量组血清PCIII、IV-C含量与增损甘露消毒丹小剂量组比较有明显差异(P<0.05)。结论增损甘露消毒丹可以抑制细胞外间质中胶原的生成,降低肝纤维化大鼠血清PCIII、IV-C的含量并能明显减轻大鼠的肝纤维化程度、减少胶原纤维与网状纤维,对四氯化碳诱导的大鼠肝纤维化有良好的治疗作用。
Objective To investigate the efficacy and mechanism of damage-enhanced mannose disinfectant against liver fibrosis. Methods Rat hepatic fibrosis model was established by subcutaneous injection of carbon tetrachloride and alcoholic gavage. Six groups were set up and each group was administered for 8 weeks. After the end of treatment, the levels of type III procollagen (PCIII) and type IV collagen (IV-C) in rat serum were detected by radioimmunoassay. Using Van-Gieson staining method and Gomori staining method, the liver fibrosis pathological grading was observed under light microscope; the degree of reticular fibrosis was analyzed by computer image analysis system. Results The levels of serum PC III and IV-C in the pathological model group were significantly higher than those in the normal group (P < 0.05). After treatment with the drug, the high-dose mannose disinfectant Dan high-dose group, the middle-dose group, and compound Biejia Ruangan Tablets were treated. The serum levels of PCIII and IV-C in the group were significantly lower than those in the pathological model group (P<0.05); the levels of PCIII and IV-C in the serum of high-dose and middle-dose groups were increased compared with the low-dose mannose-disinfection group. There was a significant difference (P<0.05). CONCLUSION: Damage-enhanced mannose disinfectant can inhibit the production of collagen in extracellular matrix, reduce the content of PCIII and IV-C in serum of hepatic fibrosis rats and significantly reduce the degree of hepatic fibrosis in rats, and reduce collagen fibers and reticular fibers. It has a good therapeutic effect on carbon tetrachloride-induced liver fibrosis in rats.