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应用 NIH纯系小鼠骨髓细胞微核试验和染色体畸变试验 ,探究海洋低温碱性蛋白酶的遗传毒性作用及对人类的潜在危害。观察并计数嗜多染红细胞 ( PCE)与正红细胞 ( NRBC)的比值、微核率 ;染色体的畸变类型和畸变率 ,检测海洋低温碱性蛋白酶的诱变作用。结果表明 ,皮下注射环磷酰胺的小鼠 ,P/ N比值 <1,微核率为 2 2 .97‰ ,染色体畸变率为 18.32 % ,与生理盐水 ( NS)对照组比较差异极显著 ( P<0 .0 1) ,而海洋低温碱性蛋白酶各组小鼠微核率均 <4‰ ,染色体畸变率为 0 .19%～0 .2 5% ,与 NS组比较无显著性差异 ( P>0 .0 5)。未见海洋低温碱性蛋白酶各组对小鼠骨髓细胞的遗传毒性。
Apply NIH pure line mouse bone marrow cell micronucleus test and chromosome aberration test to explore the genotoxicity of marine low temperature alkaline protease and its potential harm to human. The ratio of PCE to NRBC, the micronucleus rate, the type of chromosome aberration and the rate of aberration were detected and counted. The mutagenicity of marine low temperature alkaline protease was detected. The results showed that the mice in subcutaneous injection of cyclophosphamide had a P / N ratio <1, a micronucleus rate of 22.97% and a chromosome aberration rate of 18.32%, which was significantly different from that of the NS group (P <0. 01). However, the rates of micronuclei in all the mice in the low temperature alkaline protease group were <4 ‰, and the chromosome aberration rates were 0.19% -0.25%. There was no significant difference between the groups (P > 0 .0 5). No genotoxicity was observed in mouse bone marrow cells in each group of marine low temperature alkaline protease.