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从某反应器活性污泥中分离筛选出1株假单胞菌属(Pseudomonas sp.)细菌,命名为DK1,并对该菌进行脱氮特性研究.在以葡萄糖为碳源,C/N量比为5时,分别以NaNO_3和NaNO_2为氮源,二者的好氧反硝化速率为4.09 mg·(L·h)-1和4.43mg·(L·h)~(-1).以二者同时为氮源脱氮率为100%;此外,菌株DK1具有异养硝化性能,NH_4~+-N平均去除速率为2.32mg·(L·h)-1.缺氧时以NO_2~--N为氮源菌株DK1可将一系列梯度浓度NO_2~--N(约100~300 mg·L-1)在36 h内降为0.当NO_3~--N和NO_2~--N同时存在时,菌株DK1会优先利用NO_3~--N进行反硝化.同时该菌株还具有同步硝化反硝化(SND)性能,可同时去除NH_4~+-N、NO_2~--N或NH_4~+-N、NO_3~--N,30 h内脱氮率分别达95.06%和94.69%.相同时间内在NH_4~+-N、NO_2~--N和NO_3~--N三者均存在时,脱氮效果最佳,达100%.菌株DK1的高效SND及反硝化性能表明其在处理含氮废水方面有一定的潜力和应用价值.
A strain of Pseudomonas sp. Was isolated and screened from a reactor activated sludge and named as DK1, and its denitrification characteristics were studied.Using glucose as carbon source, C / N amount The aerobic denitrification rates were 4.09 mg · (L · h) -1 and 4.43 mg · (L · h) -1 with NaNO_3 and NaNO_2 as the nitrogen sources respectively, Meanwhile, the denitrification rate of nitrogen source was 100%. In addition, strain DK1 had heterotrophic nitrification ability, the average removal rate of NH_4 ~ + -N was 2.32 mg · (L · h) A series of gradient concentrations of NO 2 ~ - N (about 100 ~ 300 mg · L -1) could be reduced to 0. within 36 h when N is a nitrogen source strain DK 1. When NO 3 -N and NO 2 -N coexist , The strain DK1 would preferentially denitrify with NO_3 ~ - N. The strain also has the function of synchronous nitrification and denitrification (SND), which can simultaneously remove NH_4 ~ + -N, NO_2 ~ --N or NH_4 ~ + -N , NO_3 ~ --N, the denitrification rates reached 95.06% and 94.69% within 30 h, respectively.When the NH_4 ~ + -N, NO_2 ~ --N and NO_3 ~ The best, up to 100% .The efficient SND and denitrification performance of strain DK1 showed that it has certain potential and application value in the treatment of nitrogen-containing wastewater.