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[目的]研究化疗药物顺铂(DDP)、长春新碱(VCR)、吉西他滨(GEM)、奈达铂(NDP)、奥沙利铂(L-OHP)、氟尿嘧啶(5-Fu)、丝裂霉素(MMC)对人胃癌细胞株(SGC-7901)抑制率及化疗药物浓度和作用时间对细胞抑制率的影响。[方法]采用MTT测定法观察DDP、VCR、GEM、NDP、L-OHP、5-Fu和MMC不同浓度及作用时间对SGC-7901细胞株的抑制作用。化疗药物浓度参照其在人体血浆峰值浓度(plasma peak concentration,PPC)的0.001～1倍范围,作用时间选择24h、48h、72h。[结果]SGC-7901细胞株对不同化疗药物的敏感性存在差异,对DDP、L-OHP、MMC、NDP、5-Fu敏感,最大抑制率分别为99.5%、100%、100%、100%和97.5%;对GEM次之,最大抑制率为67.6%;对VCR不敏感。DDP、L-OHP、NDP和MMC的药物浓度与抑制率密切相关,GEM、5-Fu和MMC的药物作用时间与抑制率密切相关。[结论]不同化疗药物对人胃癌细胞株(SGC-7901)的抑制作用及量效、时效关系,为临床胃癌化疗方案的制定提供实验依据。
[Objective] To investigate the effects of chemotherapeutic agents such as DDP, VCR, GEM, NDP, L-OHP, 5-Fu, Inhibitory effect of MMC on human gastric cancer cell line (SGC-7901) and the effect of chemotherapy drug concentration and action time on the cell inhibition rate. [Method] MTT assay was used to observe the inhibitory effect of DDP, VCR, GEM, NDP, L-OHP, 5-Fu and MMC at different concentrations and time on SGC-7901 cell line. The concentration of chemotherapeutic drugs refers to its range of 0.001 to 1 times of the plasma peak concentration (PPC) in human body. The duration of action is 24 h, 48 h, 72 h. [Results] The sensitivity of SGC-7901 cell line to different chemotherapeutics was different. The sensitivity of SGC-7901 cells to DDP, L-OHP, MMC, NDP and 5-Fu was 99.5%, 100%, 100% and 100% And 97.5% respectively. For GEM, the maximum inhibition rate was 67.6%, which was not sensitive to VCR. The drug concentration of DDP, L-OHP, NDP and MMC is closely related to the inhibition rate. The drug action time of GEM, 5-Fu and MMC are closely related to the inhibition rate. [Conclusion] The inhibitory effect of different chemotherapeutic drugs on human gastric cancer cell line (SGC-7901) and its dose-response and time-effect relationship provide experimental evidence for the development of clinical gastric cancer chemotherapy regimen.