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为探讨以病小穗率与病穗率作为抗性评价指标的精确度,利用单花滴注、孢子液喷雾分别结合土表病麦粒接种的方法,评价了2个大麦重组自交系群体131个株系对赤霉病的抗侵染与抗扩展性。单花滴注接种后调查了第7、14和21天的病情性状,接种后第7天所有株系均感病,病小穗率最低的为1.59%,第21天最高病小穗率为58.91%;孢子液喷雾接种后第21天材料全部感病,其中6棱株系的感病程度高于2棱株系。以病小穗率和病穗率划分赤霉病抗性的分布情况,发现病小穗率更能有效区分株系的赤霉病抗性。相关分析显示,病小穗率、病穗率、禾谷镰刀菌烯醇含量与粒色和穗密度呈显著负相关,而与株高、抽穗期无显著相关。
In order to explore the accuracy of disease spike rate and disease panicle rate as the index of resistance evaluation, two groups of recombinant inbred lines of barley were evaluated by single instillation and spore liquid spray inoculation with the wheat planted in soil form, respectively Resistance to Fusarium head blight in 131 lines and its resistance to expansion. After single instillation inoculation, the disease traits on days 7, 14 and 21 were investigated. All the strains were susceptible on the 7th day after inoculation, with the lowest rate of disease spikelets being 1.59%. The highest rate of disease spikelets on day 21 was 58.91%. Spores were all susceptible to infection on the 21st day after spray inoculation, and the susceptibility of the six-rowed lines was higher than that of the two-rowed lines. The distribution of scab resistance was divided by disease spike rate and disease spike rate. It was found that the disease spike rate was more effective in distinguishing the scab resistance of the lines. Correlation analysis showed that there was a significant negative correlation between disease spike rate, disease panicle rate, Fusarium graminearum enol content and grain color and spike density, but not with plant height and heading date.