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目的观察亚低温对局灶性脑缺血大鼠缺血局部细胞间黏附分子-1(ICAM-1)表达和血清白细胞介素-6(IL-6)含量的影响,探讨亚低温对脑缺血性损害的神经保护作用机制。方法将30只体质量在250～300 g的雌性Sprague-Dawley大鼠随机分为亚低温组(n=15)和对照组(n=15),采用线栓法阻断大鼠一侧大脑中动脉制作局灶性脑缺血模型,制模成功后,将亚低温组和对照组大鼠分别置于冰毯机和常温操作台上,使其肛温保持在(33±1)℃和(37±0.5)℃。12 h后,自左心室取血,断头取脑,采用免疫组化方法检测缺血区ICAM-1阳性血管数目和应用免疫放射测定法(IRMA)检测血清IL-6含量。结果免疫组化分析示缺血局部ICAM-1的表达较对照组下降,IRMA检测的血清IL-6含量亚低温组较对照组降低。结论亚低温可降低局灶性脑缺血大鼠血清IL-6含量和减少缺血局部ICAM-1的表达,推测亚低温降低IL-6和ICAM-1的表达为亚低温减轻脑缺血性损害的神经保护作用之一。
Objective To investigate the effects of mild hypothermia on the expression of ICAM-1 and the level of serum interleukin-6 (IL-6) in focal cerebral ischemia rats. To investigate the effects of mild hypothermia on brain ischemia Neuroprotective mechanism of bloody damage. Methods Thirty female Sprague-Dawley rats weighing 250-300 g were randomly divided into mild hypothermia group (n = 15) and control group (n = 15) After the successful model making, the rats in the mild hypothermia group and the control group were respectively placed on the ice blanket machine and the room temperature console, the rectal temperature was kept at (33 ± 1) ℃ and ( 37 ± 0.5) ° C. After 12 h, blood was taken from the left ventricle and the brain was decapitated. The number of ICAM-1 positive blood vessels in the ischemic area was detected by immunohistochemistry and the level of IL-6 in serum was detected by immuno-radioimmunoassay (IRMA). Results Immunohistochemical analysis showed that the expression of ICAM-1 in the ischemic area was lower than that in the control group. The serum IL-6 level detected by IRMA was lower in the hypothermia group than in the control group. Conclusion Mild hypothermia can reduce the level of serum IL-6 and decrease the expression of ICAM-1 in focal cerebral ischemia rats. It is hypothesized that mild hypothermia can reduce the expression of IL-6 and ICAM-1 to reduce the cerebral ischemia One of the neuroprotective effects of damage.