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目的研究中国HIV-1高暴露持续血清阴性(highly exposed persistently seronegative,HEPS)者的Nef、Gag特异性细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)应答特点,探讨HIV-1特异性CIL应答在这类特殊人群中抵抗感染的作用机制。方法选取10例HIV-1高暴露持续血清阴性者,11例经性接触感染且从未接受抗病毒治疗的HIV/AIDS患者及4例未经暴露的健康志愿者。以覆盖HIV-1gag全长和部分nef的14个肽段库为刺激原,应用IFN-γELISPOT法测定3组人群的特异性CTL应答,并对3组的应答强度、宽度以及对肽段库识别比例进行比较。结果50％(5/10)的HEPS,100％(11/11)的HIV/AIDS患者均存在Nef及Gag特异性CTL应答,而4例健康对照均为阴性。存在应答的HEPS者对14个肽段库的平均应答强度和宽度分别是HIV/AIDS患者的4．3％和37．7％。在HEPS者中主要识别的肽段库均为HIV/AIDS患者中识别比例相对较低的肽段库。结论与HIV/ AIDS患者相比,HEPS者中的HIV-1特异性CTL应答存在着不同的特点和规律,可能在保护机体免于HIV-1感染中发挥着重要作用。
Objective To investigate the characteristics of Nef and Gag-specific cytotoxic T lymphocyte (CTL) response in highly exposed persistently seronegative (HEPS) patients in China and to explore the role of HIV-1-specific CIL response in The mechanism of action against infection in this particular population. Methods Totally 10 HIV / AIDS patients with high persistent HIV-1 seropositivity, 11 HIV / AIDS patients who had sexually transmitted infections and never received antiviral therapy, and 4 healthy volunteers who had not been exposed were selected. The specific CTL responses of three groups were determined by IFN-γELISPOT assay, and the response strength and breadth of three groups and the identification of peptide library Ratio to compare. Results Both Nef and Gag-specific CTL responses were found in 50% (5/10) of HEPS and 100% (11/11) of HIV / AIDS patients, and all four healthy controls were negative. The mean response strength and width of the 14 peptide pools to HEPS with response were 4.3% and 37.7% of HIV / AIDS patients, respectively. Peptide libraries that are predominantly identified in HEPS are libraries of relatively low recognition ratios among HIV / AIDS patients. Conclusions HIV-1-specific CTL responses in HEPS are different in characteristics and patterns compared with HIV / AIDS patients and may play an important role in protecting the body from HIV-1 infection.