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目的:观察人参皂苷Rg1诱导的神经干细胞(NSCs)在移植治疗缺氧缺血新生模型鼠中的作用。方法:体外用人参皂苷Rg1诱导分化NSCs,然后将诱导分化的NSCs移植入缺氧缺血的新生鼠模型侧脑室,采用TTC染色和行为学观察对模型进行评价。通过水迷宫、体感诱发电位观测其脑功能的恢复情况,免疫组化检测移植的NSCs生长、分化状况。结果:移植Rg1诱导后的NSCs,可以明显改善水迷宫的潜伏期、游泳路程、目标象限探索时间以及体感诱发电位的潜伏期和振幅,并在海马区域呈集中表达并围绕缺血损伤区域生长。结论:Rg1诱导后的NSCs移植在治疗新生大鼠缺氧缺血性脑损伤中发挥了较好的作用。
OBJECTIVE: To observe the role of ginsenoside Rg1-induced neural stem cells (NSCs) in neonatal rat models of hypoxia-ischemia. Methods: NSCs were induced by ginsenoside Rg1 in vitro. Then the differentiated NSCs were transplanted into the lateral ventricle of hypoxic-ischemic neonatal rat model. TTC staining and behavioral observation were used to evaluate the model. The water maze and somatosensory evoked potentials were used to observe the recovery of brain function. The growth and differentiation of transplanted NSCs were detected by immunohistochemistry. Results: Transplantation of Rg1-induced NSCs could significantly improve the labyrinth latency, swimming distance, target quadrant exploration time and the latency and amplitude of somatosensory evoked potentials. It was concentrated in the hippocampus and grew around the ischemic area. CONCLUSION: Rg1-induced NSCs transplantation plays a good role in the treatment of hypoxic-ischemic brain damage in neonatal rats.