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采用水提醇沉法制备黄芪多糖提取物,苯酚-硫酸分光光度法测定多糖的含量,在0.00—0.14mg/mL线性范围内具有良好的线性关系,回收率在94.81%—103.50%。测定黄芪多糖含量为4.3%。采用校准曲线法,用空气-乙炔火焰原子吸收光谱法测定样品中Fe、Zn、Cu和Mn的含量,膜荚黄芪中微量元素含量较高。
The polysaccharide extract of Astragalus mongholicus was prepared by water extraction and alcohol precipitation, and the content of polysaccharide was determined by phenol-sulfuric acid spectrophotometry. The linear range was 0.00-0.14mg / mL, the recoveries were 94.81% -103.50%. Determination of Astragalus polysaccharide content of 4.3%. The contents of Fe, Zn, Cu and Mn in the samples were determined by air-acetylene flame atomic absorption spectrometry with the calibration curve method. The content of trace elements in the Astragalus membranaceus was high.