论文部分内容阅读
探讨建立金黄地鼠动脉粥样硬化(AS)模型的方法,进而考察该模型病理和生化指标的变化,为该疾病的研究提供相应的试验材料。采用饲喂金黄地鼠纯化高脂饲料的方法建立AS疾病模型,通过主动脉弓的常规病理检查确定金黄地鼠疾病发展情况,并利用主成分分析(PCA)和聚类分析方法对血清生化指标进行分析。结果表明,模型组主动脉弓管壁明显增厚,管腔狭窄,内膜及中膜界限不清,并有大量的脂质积累,平滑肌细胞发生变性、坏死并呈灶性聚集。主成分分析结果将19项生化指标归为五类,分别代表血脂血糖、肾代谢、肝功、胆功及肌肉代谢相关指征。聚类分析结果与样品采集结果一致。生化指标统计结果表明,AS金黄地鼠脂质代谢紊乱,肝功和肾功相关指标差异显著。表明,通过饲喂纯化高脂饲料12周的方法能够建立金黄地鼠AS模型。血清生化指标分析结果表明,高脂饲料可导致金黄地鼠脂质代谢紊乱并可能引起肝肾功能的损伤。
To investigate the method of establishing the atherosclerosis (AS) model of golden hamster, and then to investigate the changes of the pathological and biochemical indexes of the model, and to provide the corresponding experimental materials for the study of the disease. The animal model of AS disease was established by feeding high-fat diet with golden hamster, the disease development of hamster was determined by routine pathological examination of aortic arch, and the serum biochemical indexes were analyzed by principal component analysis (PCA) and cluster analysis . The results showed that the aortic arch wall of the model group was significantly thicker, the lumen was narrow, the intima and media were not clearly defined, and there was a large amount of lipid accumulation. The smooth muscle cells degenerated, necrotic and showed focal aggregation. The results of principal component analysis classified 19 biochemical indexes into five categories, which were respectively related to the indicators of blood lipid and blood glucose, renal metabolism, liver function, biliary function and muscle metabolism. Cluster analysis results and sample collection results. Biochemical indicators of statistical results show that AS hamster dyslipidemia, liver function and kidney related indicators significant difference. Showed that hamster AS model can be established by feeding purified high-fat diet for 12 weeks. Analysis of serum biochemical indicators showed that high-fat diet can lead to lipid disturbance in golden hamster and may cause liver and kidney damage.