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目的研究NGI对转基因阿尔茨海默病小鼠的治疗作用。方法构建包含小鼠OMgp细胞外8LRP重复序列,人Tenascin-R的EGF-L结构域,以及人Nogo-A的氨基端和66个氨基酸细胞外域的重组质粒,肌肉注射AD小鼠,空载体组和空白对照组为对照组。水迷宫检测小鼠行为学差别,免疫组化检测其病理差别。结果4.5月龄AD小鼠经过水迷宫隐蔽平台实验,2d后疫苗组小鼠潜伏期比对照组缩短30%(P<0.05)。探索实验显示3月龄疫苗组比空载体组和空白对照组小鼠穿越目标象限的次数分别增加48%和44%(P<0.05)。结论NGI重组DNA疫苗能够提高转基因AD小鼠的学习与记忆能力。
Objective To study the therapeutic effect of NGI on transgenic Alzheimer’s disease mice. Methods The recombinant plasmid containing mouse OMgp extracellular 8LRP repeats, human Tenascin-R EGF-L domain and human Nogo-A amino terminal and 66 amino acid extracellular domain was constructed and intramuscularly injected with AD mice. And blank control group as control group. Water maze test mice behavioral differences, immunohistochemistry to detect the pathological differences. Results The AD mice aged 4.5 months passed the water maze concealed platform experiment. After 2 days, the mice in the vaccine group had a 30% shorter latency (P <0.05) than the control group. Exploration experiments showed that the numbers of crossing the target quadrant of mice in the 3-month-old vaccine group increased by 48% and 44% (P <0.05) respectively compared with the empty vector group and the blank control group. Conclusion NGI recombinant DNA vaccine can improve the learning and memory ability of transgenic AD mice.