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目的研究分析我院皮肤性病门诊患者生殖支原体(Mg)的感染情况。方法收集2015年2—10月就诊于皮肤性病门诊疑似泌尿生殖系统感染的患者235例(男165例,女70例),采集男性尿道或女性宫颈分泌物分别进行超显微镜检、淋球菌培养、支原体培养、生殖道沙眼衣原体聚合酶链反应(PCR)检测和生殖支原体实时荧光核酸恒温扩增检测技术(SAT)检测。结果 235例患者中各种微生物检出情况分别为Mg 17例(7.23%),解脲脲原体(Uu)72例(30.64%),人型支原体(Mh)22例(9.36%),生殖道沙眼衣原体(Ct)10例(4.26%),淋球菌6例(2.55%),上述检测项目全部阴性122例(51.91%)。Mg阳性的17例患者中单一感染7例(41.18%),混合感染10例(58.82%),其中Mg+Uu感染6例,Mg+Uu+Mh感染4例。结论 Mg可单一感染,也可混合感染,临床上应加强对性病门诊患者Mg的筛查。Mg生长条件要求高,培养成功率低,SAT检测是目前可以选择用来检测Mg的方法之一。
Objective To analyze and analyze the infection of Mycoplasma genitalium (Mg) in patients with dermatosis clinic in our hospital. Methods A total of 235 patients (165 males and 70 females) with suspected genitourinary tract infections in dermatology clinics from January to October 2015 were collected. Male urethra or female cervical secretions were collected for microscopic examination, gonococcal culture, Mycoplasma, genital tract Chlamydia trachomatis polymerase chain reaction (PCR) detection and Mycoplasma genitalium real-time fluorescent nucleic acid thermostatic amplification detection (SAT) detection. Results The detection of various microorganisms in 235 cases were 17 cases (7.23%) of Mg, 72 cases (30.64%) of Uu, 22 cases (9.36%) of Mh, There were 10 cases (4.26%) of C. trachomatis and 6 cases (2.55%) of Neisseria gonorrhoeae, all of which were negative in 122 cases (51.91%). Seventeen patients (41.18%) were single infection and 10 (58.82%) were mixed infections, among which six were Mg + Uu infection and four were Mg + Uu + Mh infection. Conclusion Mg can be single infection, but also mixed infection, clinical screening of Mg in STD clinics should be strengthened. Mg growth conditions require high, low success rate of culture, SAT detection is one of the methods that can be selected to detect Mg.