微流控芯片上电驱动在线富集技术是一种有效提高分析效率、检测灵敏度和降低对检测器要求的技术和方法。本文针对目前微流控芯片分析系统中生化样品的预处理问题,对芯片上电驱动在线富集技术进行了分析讨论,介绍了等速电泳、等电聚焦、场放大和介电电泳的样品预富集技术在微流控芯片上的实现与应用,并对每一种技术的原理、特点、存在的问题、近年发展的状况和发展趋势进行了综述。 Microfluidic chip on-line power-driven enrichment technology is an effective method to improve the analysis efficiency, detection sensitivity and reduce the requirements of the detector. In this paper, in order to solve the problem of pretreatment of biochemical samples in microfluidic chip analysis system, the on-chip on-chip electrokinetic enrichment technology was analyzed and discussed. The sample pretreatment including isokinetic electrophoresis, isoelectric focusing, field amplification and dielectrophoresis The realization and application of enrichment technology in microfluidic chips are reviewed. The principle, characteristics, existing problems, the status of development and the development trend of each technology are summarized.