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采用一组系列相关单克隆抗体和流式细胞术间接免疫荧光法分析 85例初治儿童急性淋巴细胞白血病 (ALL)患者的免疫表型 ,观察儿童ALL髓系抗原表达及其与临床和生物学特性的关系。研究结果表明 ,儿童ALL髓系抗原阳性率达 2 1.2 % ,以CD13和CD33阳性常见。T系ALL与B系ALL髓系抗原表达无差异 (P >0 .75 )。T/B混合ALL中髓系抗原表达率较高 (7例中 3例阳性 )。ALL L2 与ALL L1髓系抗原表达无差异 (P >0 .0 5 ) ;髓系抗原阳性ALL与髓系抗原阴性ALL临床生物学特征、染色体数量及其结构变化均无差异 (P >0 .2 5 ) ;髓系抗原阳性病例完全缓解 (CR)率低于阴性病例 ,1年内复发率高于阴性病例 ,但均无差异 (P >0 .0 5 )。结论提示 ,儿童ALL髓系抗原表达与治疗缓解率无关 ;T/B混合ALL的髓系抗原的表达较高 ,且预后不良
A series of monoclonal antibodies and flow cytometry indirect immunofluorescence method was used to analyze the immunophenotype of 85 children with acute lymphoblastic leukemia (ALL) and to observe the expression of ALL myeloid antigen in children with clinical and biological Characteristics of the relationship. The results show that children with myeloid antigen positive rate of 2 1.2%, with CD13 and CD33 positive common. There was no difference in myeloid antigens between T lineage ALL and B line (P> 0.75). The myeloid antigen expression was higher in T / B mixed ALL (positive in 3 of 7). There was no difference in the expression of ALL L2 and ALL L1 myeloid antigens (P> 0.05). There was no significant difference in the biological characteristics, number of chromosomes and their structural changes among myeloid antigen-positive ALL and myeloid-negative ALL patients (P> 0.05). The complete remission (CR) rate of myeloid antigen positive cases was lower than that of negative cases, and the recurrence rate was higher in 1 year than in negative cases (P> 0.05). The results suggest that the expression of myeloid antigens in children with ALL has no relation with the remission rate; the myeloid antigens of T / B mixed ALL have a higher expression and the prognosis is poor