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研究初始pH值、木糖和葡萄糖配比以及温度对F11乙醇发酵的影响并对其进行发酵抑制剂耐受试验。结果表明,发酵最适初始pH值为4.0~7.0,葡萄糖和木糖最适配比为0%~33%,温度为24~27℃。在初始pH值5.0、转速150 r/min、培养温度27℃、木糖45 g/L、葡萄糖15 g/L,接种量1g/L的条件下发酵,乙醇浓度最高可达28g/L,达到理论产率的91%。添加乙酸明显降低了融合子F11的乙醇发酵速率,模拟预处理后水解液添加葡萄糖醛酸(2.0 g/L)、甲酸(0.9 g/L)、糠醛(0.1g/L)和乙酸(2.3g/L),其协同抑制作用使发酵24 h内几乎无乙醇产生。
The effects of initial pH value, xylose and glucose ratio and temperature on F11 ethanol fermentation were studied and the fermentation inhibitor tolerance test was carried out. The results showed that the optimal initial pH was 4.0-7.0, the optimal ratio of glucose to xylose was 0% -33%, and the temperature was 24-27 ℃. Under the conditions of initial pH 5.0, rotation speed 150 r / min, culture temperature 27 ℃, xylose 45 g / L, glucose 15 g / L and inoculum 1 g / L, the highest ethanol concentration was 28 g / The theoretical yield of 91%. The addition of acetic acid significantly reduced the ethanol fermentation rate of the fusion F11. After the simulated pretreatment, the glucuronic acid (2.0 g / L), formic acid (0.9 g / L), furfural (0.1 g / L) and acetic acid / L), the synergistic inhibition of fermentation within 24 h almost no ethanol.