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目的讨论不同浓度胰升血糖素(Glg)及葡萄糖(Glu)对MIN6胰岛β细胞株胰岛素分泌和细胞增殖活力的影响。方法采用不同浓度的Glu(2.8、16.7mmol/L)及Glg(100、500、1000ng/L)处理MIN6细胞,ELISA测定细胞上清液中胰岛素的含量,MTT法测定各组细胞增殖活力,收集整理实验数据并进行统计分析。结果 (1)胰岛素含量:单纯Glu处理后,16.7mmol/L组高于2.8mmol/L组(P<0.001);单纯Glg处理后,1000ng/L组高于100ng/L组和500ng/L组(P<0.01或P<0.001);2.8mmol/L Glu加不同浓度Glg处理后,2.8mmol/L Glu+1000ng/L Glg组高于2.8mmol/L Glu+100ng/L Glg组和2.8mmol/L Glu+500ng/L Glg组(P<0.001),2.8mmol/L Glu+500ng/L Glg组高于2.8mmol/L Glu+100ng/L Glg组(P<0.001);16.7mmol/L Glu加不同浓度Glg处理后,16.7mmol/L Glu+1000ng/L Glg组高于16.7mmol/L Glu+100ng/L Glg组和16.7mmol/L Glu+500ng/L Glg组(P<0.001),16.7mmol/L Glu+500ng/L Glg组高于16.7mmol/L Glu+100ng/L Glg组(P<0.001);(2)细胞增殖活力:2.8mmol/L Glu加不同浓度Glg处理后,各组细胞活力变化比较,差异无统计学意义(P>0.05);生理浓度Glu(5.6 mmol/L)加不同浓度Glg处理后,与无Glg组比较,其他Glg组增高[(0.36±0.06)vs(0.50±0.06),(0.59±0.10),(0.54±0.03)ng/L,P<0.05或P<0.01];16.7mmol/L Glu加不同浓度Glg处理后,与16.7mmol/L Glu+1000ng/L Glg组比较,16.7mmol/L Glu+100ng/L Glg、16.7mmol/L Glu+500ng/L Glg及无Glg组降低[(0.94±0.15)vs(0.66±0.06),(0.68±0.14),(0.68±0.03)ng/L,P<0.01]。结论 Glg可能通过葡萄糖依赖的方式促进MIN6胰岛β细胞株胰岛素分泌,并增加细胞的增殖活力。
Objective To investigate the effects of different concentrations of glucagon (Glg) and glucose (Glu) on the insulin secretion and cell proliferation of MIN6 islet β cell line. Methods MIN6 cells were treated with Glu (2.8, 16.7mmol / L) and Glg (100, 500, 1000ng / L) at different concentrations. The contents of insulin in the supernatant were measured by ELISA. Organize the experimental data and conduct statistical analysis. Results: (1) The content of insulin in the group of Glutamic acid was higher than that in the group of 16.7mmol / L and 2.8mmol / L (P <0.001) (P <0.01 or P <0.001). After treatment with 2.8 mmol / L Glu and Glg at different concentrations, Glutamine 2.8 mmol / L Glu + 1000 ng / L Glg group was higher than 2.8 mmol / L Glu + 100 ng / L Glu +500 ng / L Glg group (P <0.001), 2.8 mmol / L Glu +500 ng / L Glg group was higher than 2.8 mmol / L Glu +100 ng / After treatment with Glg at different concentrations, Glutamic acid levels of 16.7 mmol / L Glu + 1000 ng / L Glg group were significantly higher than those of Glutamine group (16.7 mmol / L Glu + 100 ng / L Glg group and 16.7 mmol / L Glu + 500 ng / / L Glu + 500ng / L Glg group was higher than that of 16.7mmol / L Glu + 100ng / L Glg group (P <0.001); (2) Cell proliferation activity: 2.8mmol / L Glu treated with different concentrations of Glg, (0.36 ± 0.06) vs (0.50 ± 0.06) vs Glg group (P <0.05). Compared with Glg group, Glu (5.6 mmol / L) and Glg treatment group showed no significant difference ± 0.06), (0.59 ± 0.10) and (0.54 ± 0.03) ng / L respectively, P <0.05 or P <0.01]. After treatment with 16.7 mmol / L Glu and Glg at different concentrations, Glg group, 16.7 mmol / L Glu + 100 ng (0.94 ± 0.15) vs (0.66 ± 0.06), (0.68 ± 0.14) and (0.68 ± 0.03) ng / L, respectively, P <0.01 ]. Conclusion Glg may promote the insulin secretion of MIN6 islet β cell line by glucose-dependent manner and increase the cell proliferation activity.