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目的:观察不同浓度葡萄糖PDS(PDS)对单层人腹膜间皮细胞(HPMCs)跨细胞电阻(TER)以及迁移修复能力的影响。方法:用不同葡萄糖浓度PDS(1.5%,2.5%,4.25%)分别与DMEM以1:1比例混合后体外培养HPMCs。四甲基偶氮唑盐(MTT)比色法测定细胞增殖。使用双池培养皿(transwell)构建HPMCs单层细胞模型。采用TER技术测定PDS对HPMCs通透性的影响。划痕损伤实验观察葡萄糖对HPMCs修复再生能力的影响。结果:不同浓度葡萄糖PDS(1.5%,2.5%,4.25%)干预均可明显地抑制HPMCs的增殖(P<0.05)。TER值随PDS干预时间的延长而逐渐下降,且与葡萄糖浓度呈负相关(P<0.01)。葡萄糖PDS可明显抑制HPMCs迁移修复能力,且与葡萄糖浓度相关。结论:高糖PDS液抑制细胞增殖,降低单层HPMCs的TER值,并抑制HPMCs损伤后迁移修复能力。提示PDS中高糖成分是导致腹透患者腹膜高通透性和超滤衰竭的重要因素。
OBJECTIVE: To observe the effects of different concentrations of PDS on the transmembrane resistance (TER) and migration and repair ability of monolayer human peritoneal mesothelial cells (HPMCs). Methods: HPMCs were cultured in vitro with PDS (1.5%, 2.5%, 4.25%) mixed with DMEM in a ratio of 1: 1. Tetramethylzole (MTT) colorimetric assay for cell proliferation. A single cell model of HPMCs was constructed using a doublewell culture vessel (transwell). The Effect of PDS on the Permeability of HPMCs Using TER Technique. Effects of glucose on repair and regeneration ability of HPMCs were observed by scratch injury test. Results: PDS (1.5%, 2.5%, 4.25%) treated with different concentrations of glucose significantly inhibited the proliferation of HPMCs (P <0.05). The TER value decreased gradually with the prolongation of PDS intervention, and was negatively correlated with glucose concentration (P <0.01). Glucose PDS can significantly inhibit HPMCs migration and repair capacity, and glucose concentration related. Conclusion: High glucose PDS solution can inhibit cell proliferation, reduce the TER value of single-layer HPMCs and inhibit the ability of migration and repair of HPMCs after injury. Tip PDS in high-sugar composition is caused by peritoneal peritoneal hyperpermeability and ultrafiltration failure an important factor.