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目的 探讨活化巨噬细胞培养调理液内的炎性细胞因子水平及其在诱发炎性 PVR模型中的作用。方法 收集兔活化巨噬细胞培养上清调理液 ,采用双抗体夹心法 EL ISA检测试剂盒 ,检测其 TNF-α、IL - 8、IL - 6的含量 ,并进行多元回归拟合曲线分析。结果 巨噬细胞体外培养上清液中 IL - 8、TNF-α及 IL - 6的含量分别于培养后的 8、2 0、48h明显增加 (分别为 12 2 pg/ml± 31pg/ml、12 5 pg/ml± 2 2 pg/m l和 2 6 pg/ml± 4pg/ml,P<0 .0 1) ,前两种细胞因子于2 0、72 h达高峰 (分别为 192 pg/ml± 38pg/m l,15 4pg/m l± 16 pg/ml,P<0 .0 1)。IL- 6至 96 h仍呈上升状态 (2 8pg/ml±4pg/ml)。结论 注入活化巨噬细胞或其细胞培养调理液中炎源细胞因子在其启动、调控 PVR的发展中起重要作用
Objective To investigate the level of inflammatory cytokines in activated macrophage culture fluid and its role in inducing inflammatory PVR model. Methods The supernatant conditioned medium of rabbit macrophage - activated macrophages was collected and the levels of TNF - α, IL - 8 and IL - 6 were detected by EL ISA kit. The multiple regression curve was analyzed. Results The levels of IL - 8, TNF - α and IL - 6 in supernatant of macrophages were significantly increased at 12, 20 and 48 h (12 2 pg / ml ± 31 pg / ml, 12 5 pg / ml ± 2 2 pg / ml and 26 pg / ml ± 4 pg / ml, P <0.01). The first two cytokines peaked at 20 h and 72 h (192 pg / ml ± 38 pg / ml, 15 4 pg / ml ± 16 pg / ml, P <0.01). IL-6 to 96 h is still rising (28pg / ml ± 4pg / ml). Conclusion Inflammatory cytokines injected into activated macrophages or their cell culture regulators play an important role in the initiation and regulation of PVR development