论文部分内容阅读
目的:从基因水平上探讨运动性心脏重塑的发生机制。方法:70只SD大鼠随机分为对照组和实验组,实验组进行75天的跑台耐力训练。分别于训练第3、10、23、37和75天次日晨宰杀实验组大鼠,每次7只。相应对照组亦于当天上午取材。以异硫氰酸胍法提取大鼠心室肌组织总RNA,聚合酶链式反应技术(PCR)测定心室肌降钙素基因相关肽(Calcitonin Gene Related Peptide,CGRP)mRNA的表达量,β-actin为内参照,CGRP mRNA/β-actin的比值为每个测试样本中CGRPmRNA的表达值。结果:在5个时相点中,实验组大鼠心室肌组织CGRP mRNA表达量与对照组相比无显著差异。结论:运动性心脏重塑过程中,大鼠心室肌组织CGRP mRNA表达量无明显变化。
Objective: To explore the mechanism of exercise-induced cardiac remodeling at the gene level. Methods: Seventy SD rats were randomly divided into control group and experimental group. The experimental group received 75 days treadmill training. Rats in experimental group were sacrificed on the morning of the 3rd, 10th, 23th, 37th and 75th day of training respectively, each time 7 rats. The corresponding control group also drawn on the morning of the same day. The total RNA was extracted from rat ventricular myocytes by guanidine isothiocyanate method. The mRNA expression of calcitonin gene-related peptide (CGRP) was measured by polymerase chain reaction (PCR). The expression of β-actin For internal reference, the ratio of CGRP mRNA / β-actin was the expression of CGRP mRNA in each test sample. Results: At 5 time points, the expression of CGRP mRNA in ventricular muscle of rats in experimental group showed no significant difference compared with control group. CONCLUSION: During the process of exercise-induced cardiac remodeling, the expression of CGRP mRNA in rat ventricular myocytes did not change significantly.