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目的 分析我国肝病患者和健康人群TTV基因型。方法 用巢式PCR扩增我国不同地区不明病因的急性肝炎、慢性肝炎、肝硬化、肝癌和自然健康人群TTV DNA片段，并对PCR产物进行克隆，测序分析和同源性比较。结果 武汉WH1、WH2、WH3 3个病人血清中TTV DNA序列与N22同源性分别为97%、97%和98%；广州GZ 1、GZ2、GZ3 3例病人血清中TTV DNA 序列与N22同源性分别为98%、95%和95%；山东SD2、SD3 TTV DNA序列与N22同源性分别为94.6%和95.5%；广州GZ4、山东SD1、新疆XJ1病人TTV DNA序列与TXO11同源性分别为98%、98%和95%。结论 根据 Okamoto分类方法，我国TTV属于两个亚型，即基因1型中的a和b亚型。
Objective To analyze the genotypes of TTV in patients with liver disease and healthy people in China. Methods Nested PCR was used to amplify TTV DNA fragments of acute hepatitis, chronic hepatitis, cirrhosis, hepatocellular carcinoma and natural healthy people with unknown etiology in different areas in China. The PCR products were cloned, sequenced and compared with homology. Results The homologies of TTV DNA sequences and N22 in serum of WH1, WH2 and WH3 patients in Wuhan were 97%, 97% and 98%, respectively. The serum TTV DNA sequences of GZ1, GZ2 and GZ3 in Guangzhou were homologous to N22 The homologies of TTV DNA sequences and N22 in Shandong SD2 and SD3 were 94.6% and 95.5%, respectively. The homologies of TTV DNA sequences and TXO11 in Guangzhou GZ4, Shandong SD1 and Xinjiang XJ1 patients were respectively 98%, 98% and 95%. Conclusion According to the Okamoto classification method, TTV belongs to two subtypes in our country, namely the genotypes a and b subtypes.