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利用傅里叶变换红外光谱仪及带ATR探头的中红外光纤系统测定了手术切除的胃癌、结直肠癌及相应的正常组织共31对标本粘膜面的反射红外光谱.结果表明,与正常组织相比,癌组织的红外光谱发生明显变化:(1)与脂类相关的谱带2955,2920,2870,2850和1740cm-1出现几率明显低于正常组织(P<0.001),I1460/I1400(I为峰强度)明显降低(P<0.001),表明癌组织的脂类相对含量降低;(2)与蛋白质相关谱带N—H和O—H明显红移(P=0.025),表明N—H和O—H的氢键化程度增加,癌组织的I3375/I1460,I1640/I1460和I1550/I1460明显升高(P<0.01),表明癌组织的蛋白质相对于脂类的含量增加,癌组织的HW1550/I1550(HW为半高宽)明显升高(P=0.036),HW1550则明显降低(P=0.05),表明癌组织中蛋白质的二级结构发生显著变化;(3)与醣类相关谱带中癌组织的I1160/I1460降低(P=0.002),结合组织化学染色,推测可能是癌组织表面的糖蛋白明显减少造成的,而I1120/I1460升高(P=0.019)则可能是癌组织表面的糖原颗粒增加所致.(4)与核酸相关谱带中癌组织的PO的反对称伸缩振动蓝移(P=0.033),表明癌组织中磷酸基团的氢键化程度降低.研究结果表明,红外光谱有望成为诊断恶性肿瘤的有力工具.
The Fourier transform infrared spectroscopy (FTIR) and mid-infrared fiber system with ATR probe were used to determine the reflectance spectra of 31 specimens of gastric cancer, colorectal cancer and corresponding normal tissues. The results showed that compared with normal tissues (1) The incidences of lipid-related bands 2955, 2920, 2870, 2850 and 1740 cm-1 were significantly lower than those of normal tissues (P <0.001), I1460 / I1400 (P <0.001), indicating that the relative content of lipids in cancerous tissues decreased; (2) the red shifts of N-H and O-H in protein bands (P = 0.025) The degree of hydrogen bonding of O-H increased. The levels of I3375 / I1460, I1640 / I1460 and I1550 / I1460 in cancerous tissues were significantly higher than those in normal tissues (P <0.01) / I1550 (HW = FWHM) was significantly higher (P = 0.036) and HW1550 was significantly lower (P = 0.05), indicating a significant change in the secondary structure of proteins in cancer tissues; (3) The reduction of I1160 / I1460 in carcinoma tissue (P = 0.002) combined with histochemical staining suggested that the glycoproteins on the surface of cancerous tissue may be significantly reduced , While the increase of I1120 / I1460 (P = 0.019) may be due to the increase of glycogen granules on the surface of cancer tissue. (4) The antisymmetric stretching vibration of PO with cancerous tissues in the nucleic acid bands was blue shift (P = 0.033 ), Indicating a decrease in the degree of hydrogen bonding of the phosphate groups in the cancer tissue.The results show that infrared spectroscopy is expected to become a powerful tool for the diagnosis of malignant tumors.