论文部分内容阅读
目的:研究补体在百草枯(PQ)急性中毒肺损伤中的作用。方法:采用20 mg/kg PQ染毒小鼠,染毒后0 h、4 h、12 h、24 h、48 h检测血清C3c水平、肺组织C3、C5a和C1q沉积和分布;选用PQ染毒剂10 mg/kg、20 mg/kg分别在染毒前48 h、36 h、24 h腹腔内注射眼镜蛇毒因子(CVF)2.5μg/200μl,染毒后48 h留取支气管肺泡灌洗液(BALF)、肺组织,与未注射CVF以相同剂量染毒小鼠比较BALF总蛋白浓度、髓过氧化物酶(MPO)活性、组织病理以及存活率差异。结果:20 mg/kg PQ染毒小鼠血清C3c水平在染毒后12 h升高,24 h达到高峰,持续至48 h;免疫组化显示染毒后4 h C3在肺组织沉积,12 h开始C3沉积减少;C5a、C1q自染毒后4 h开始在肺组织沉积,且随病程延长,着色逐渐加重;CVF阻断补体后染毒小鼠肺组织损伤减轻,炎性细胞浸润减少,且存活时间显著延长。结论:在小鼠PQ中毒急性肺损伤早期,补体即被激活,并发挥重要作用,采用CVF进行补体抑制或阻断,可以有效减轻组织病理损伤。
Objective: To study the role of complement in acute lung injury induced by paraquat (PQ). Methods: Serum levels of C3c, C3, C5a and C1q in lung tissue were measured at 0, 4, 12, 24 and 48 h after exposure to 20 mg / kg PQ. 10 μg / kg and 20 mg / kg of cobra venom factor (CVF) 2.5 μg / 200 μl were intraperitoneally injected at 48 h, 36 h and 24 h before exposure to bronchoalveolar lavage fluid (BALF ), Lung tissue, BALF total protein concentration, myeloperoxidase (MPO) activity, histopathology and survival rate were compared in mice exposed to the same dose of untreated CVF. Results: Serum levels of C3c in 20 mg / kg PQ-treated mice increased at 12 h and reached the peak at 24 h after exposure to 48 h. Immunohistochemistry showed that C3 at 4 h after lung injury was deposited at 12 h Began to C3 deposition decreased; C5a, C1q 4 h after the start of the deposition in the lung tissue, and with the prolonged course of the coloring gradually aggravated; CVF blocked complement post-infection mice lung tissue damage reduced inflammatory cell infiltration, and Survival time significantly longer. Conclusion: In the early stage of PQ poisoning acute lung injury, complement is activated and plays an important role. Complement inhibition or blockade with CVF can effectively reduce the histopathological damage.