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为了研究细胞活化后组蛋白磷酸化程度的改变以及对其免疫原性的影响。将正常淋巴细胞组蛋白人为加磷酸、活性组蛋白人为去磷酸 ,用Westernblot法检测其磷酸化程度 ;然后用以上不同组蛋白同系免疫BALB/c小鼠 ,用ELISA法测定IgG类抗组蛋白抗体、抗dsDNA抗体 ,用免疫荧光法检测抗核抗体核型及肾脏病理 ,用免疫印迹法测定可溶性核抗原抗体。结果发现淋巴细胞活化后 ,其组蛋白H1成分发生明显的丝氨酸磷酸化 ,对正常组蛋白人为加磷酸仍无免疫原性 ,活性组蛋白人为去磷酸后仅影响抗体产生的滴度 ,仍具有免疫原性。所以丝氨酸磷酸化在组蛋白的免疫原性改变方面起一定的作用 ,但不是主要因素
In order to study the changes of the degree of histone phosphorylation after cell activation and its influence on the immunogenicity. Normal human lymphocyte histones were phosphorylated and the activity of histone was dephosphorylated. The phosphorylation of human histone was determined by Western blot. The BALB / c mice were immunized with different histones, and IgG anti-histone antibodies , Anti-dsDNA antibody, anti-nuclear antibody karyotype and renal pathology were detected by immunofluorescence and soluble nuclear antigen antibody was detected by immunoblotting. The results showed that the activation of lymphocytes, the histone H1 component of significant serine phosphorylation of normal histone human add phosphoric acid is still no immunogenicity, the activity of human histone dephosphorylation only affect antibody titer, still with immunity Original. Therefore, serine phosphorylation plays a role in the immunogenicity of histones but is not a major factor