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目的观察仙鹤草药物血清对人非小细胞肺癌细胞株A549增殖、形态、凋亡的影响,验证仙鹤草的体外抗肿瘤作用。方法不同方法制备仙鹤草含药血清,分别作用于A549细胞,通过CCK8的方法考察细胞增殖情况,选出最佳方法的仙鹤草含药血清;倒置显微镜观察最佳方法的仙鹤草含药血清对A549细胞形态的影响,并用Annexin V-FITC/PI双染流式细胞术考察其诱导细胞凋亡的作用。结果两种仙鹤草含药血清对A549细胞均有增殖抑制作用,与正常对照组比较其中含药血清Ⅱ效果更为明显(P<0.01),且能够促进细胞的早期凋亡和坏死,降低其存活率。结论仙鹤草含药血清有一定的体外抗肿瘤细胞生长作用,该作用受制备方法、剂量等因素影响。
Objective To observe the effect of agrimony serum on the proliferation, morphology and apoptosis of human non-small cell lung cancer cell line A549 and to verify its anti-tumor effect in vitro. Methods Different methods were used to prepare Agrimonia sera containing A549 cells. A549 cells were treated with CCK8, and the best agar serum was selected by CCK8 assay. The best agar containing sera A549 cells. The apoptosis of A549 cells was detected by Annexin V-FITC / PI double staining flow cytometry. Results Both Agrimonia senticosus serum had inhibitory effects on the proliferation of A549 cells. Compared with the normal control group, the drug-containing serum Ⅱ showed more obvious effect (P <0.01), and could promote the early apoptosis and necrosis of cells, Survival rate. Conclusion Agrimonia senticosus serum has anti-tumor cell growth in vitro, which is affected by the preparation methods, dosage and other factors.