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目的:观察日本血吸虫(大陆株)次黄嘌呤鸟嘌呤磷酸核糖转移酶(hypoxanthine-guanine phosphoribosyl-transferase,HGPRT)重组抗原(reSjc HGPRT)与ISA206或弗氏佐剂联合免疫对小鼠诱导抗日本血吸虫感染的保护作用。方法:雌性C57BL/6小鼠随机分为5组:reSjc HGPRT加ISA206佐剂免疫组、reSjc HGPRT加弗氏佐剂组、ISA206佐剂对照组、弗氏佐剂对照组和感染对照组。20μg重组抗原和ISA206或弗氏佐剂乳化后小鼠项背部多点皮下注射,共免疫3次,每次间隔2周。佐剂对照组小鼠仅注射ISA206或弗氏佐剂和生理盐水,感染对照组不注射任何重组抗原或生理盐水。于末次免疫后3周,每只小鼠经腹部皮肤感染(30±1)条日本血吸虫尾蚴,6周后剖杀小鼠,门脉灌注收集成虫,计数成虫数、雌雄合抱数和小鼠肝组织虫卵数。在免疫前、攻击感染前和小鼠剖杀前分别采血并分离血清,用ELISA检测血清中特异性IgG抗体。结果:重组抗原加ISA206佐剂或弗氏佐剂免疫组均诱导小鼠产生特异性IgG抗体应答,与感染对照组和弗氏佐剂对照组相比,差异有统计学意义(P<0.05);诱导小鼠产生的减虫率、减雌雄合抱率和肝组织减卵率分别为53.7%、59.3%、44.9%和43.3%、44.1%、33.0%,与感染对照组和2种佐剂对照组相比均有统计学意义(P<0.05)。结论:reSjcHGPRT与ISA206或弗氏佐剂联合免疫,可诱导小鼠产生抗血吸虫感染保护作用。
OBJECTIVE: To observe the anti-Schistosoma japonicum resistance induced by combined immunization with reSjc HGPRT and ISA206 or Freund’s adjuvant in Schistosoma japonicum (Continent strain) Infection protection. Methods: Female C57BL / 6 mice were randomly divided into 5 groups: reSjc HGPRT plus ISA206 adjuvant immunization group, reSjc HGPRT plus Freund’s adjuvant group, ISA206 adjuvant control group, Freund’s adjuvant control group and infection control group. 20μg recombinant antigen and ISA206 or Freund’s adjuvant emulsified mouse back subcutaneous injection, co-immunization three times, each interval of two weeks. Adjuvant control mice were injected only with ISA206 or Freund’s adjuvant and saline, and infected controls did not receive any recombinant antigen or saline. Three weeks after the last immunization, mice were infected with cercariae of Schistosoma japonicum (30 ± 1) via abdomen skin. After 6 weeks, mice were sacrificed and adult mice were collected by portal vein perfusion. The number of adult worms, Tissue egg number. Serum samples were collected before immunization, before infection and before the mice were sacrificed. Serum samples were tested for serum IgG by ELISA. Results: The recombinant antigens plus ISA206 adjuvant or Freund’s adjuvant immunization group induced specific IgG antibody response in mice, which was significantly different from the control group and Freund’s adjuvant control group (P <0.05) ; The worm reduction rate induced by the mice was decreased by 53.7%, 59.3%, 44.9% and 43.3%, 44.1% and 33.0% respectively, compared with the control group and two adjuvants Group were statistically significant (P <0.05). Conclusion: Combined immunization with reSjcHGPRT and ISA206 or Freund’s adjuvant can induce anti-schistosome infection in mice.