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目的 考察含药血清方法的可靠性。方法 自 1d龄 SD大鼠四肢长骨获得破骨细胞 ,接种于象牙薄片上 ,于培养第 7d取出 ,计数各组骨吸收陷窝的数量。选用具有明显抑制破骨细胞骨吸收活性作用的西药福善美、固邦和倍美力 ,以等效剂量给大鼠灌胃后制备含药血清 ,分别与相应药物直接添加的 3个浓度 (1× 10 - 8M、 1× 10 - 6 M、 1× 10 - 4 M)组进行比较 ,观察骨吸收陷窝数量的变化。同等条件下制备生理盐水灌胃对照血清。结果 3种药物含药血清及其直接添加各组的骨吸收陷窝数量均显著性地低于对照组。其中 ,福善美含药血清组与直接添加的 3个浓度组之间无显著性差异 (P>0 .0 5 ) ,固邦和倍美力含药血清组与 10 - 8M直接添加组之间无显著性差异 (P>0 .0 5 ) ,但作用强度不及 10 - 6 M和 10 - 4 M直接添加组 (P<0 .0 5或 P<0 .0 1)。结论 含药血清方法具有一定的可靠性 ,本实验条件下制备的含药血清 ,其药效强度大致相当于直接添加的 10 - 8M浓度。
Objective To investigate the reliability of the drug-containing serum method. Methods Osteoblasts were obtained from the long bones of SD rats of 1 day old SD rats and were inoculated on ivory slices. They were taken out on the 7th day of culture, and the number of resorption lacunae in each group was counted. Western medicine Fosamax, Solid State, and Premarin were used to inhibit bone resorption activity of osteoclasts. Rats were given gavage at the equivalent dose, and 3 concentrations were directly added to the corresponding drug (1× The 10-8M, 1×10-6 M, and 1×10-4 M groups were compared to observe changes in the number of bone resorption lacunae. Under the same conditions, normal saline was administered to the control serum. Results The number of bone resorption lacunas in the 3 drug-containing serum and directly added to each group were significantly lower than those in the control group. Among them, there was no significant difference between Fushanmei-containing serum group and the three concentrations directly added (P>0.05). There was no difference between Fubangmei-containing serum group and 10-8M direct-addition group. Significant difference (P>0.05), but the effect was less than 10-6 M and 10 -4 M added directly (P<0.05 or P<0.01). Conclusions The drug-containing serum method has certain reliability. The drug-containing serum prepared under the experimental conditions has a potency equivalent to the directly added 10-8 M concentration.