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目的探讨骨化三醇对糖尿病大鼠股骨力学结构的影响。方法链脲佐菌素诱导建立糖尿病大鼠模型,随机分为糖尿病组(DM)和骨化三醇灌胃组(VD),健康同龄大鼠作为正常对照组(NC)。骨化三醇灌胃后6周、9周,取右侧股骨做生物力学检测;取左侧股骨制备脱钙骨切片,进行骨形态学观察和计量学测定。结果与同期NC组相比,各DM组大鼠股骨的生物力学参数均明显下降(Ρ<0.05),组织形态计量学参数显示骨小梁数量、骨小梁厚度和骨小梁面积百分比均明显下降(Ρ<0.05),而骨小梁分离度明显增加(Ρ<0.05)。骨化三醇灌胃后6周,与同期DM组相比,大鼠股骨的生物力学参数显示最大载荷(132.47±11.00)和断裂载荷(115.37±7.64)均明显增加(Ρ<0.05),形态学参数显示骨小梁数量(3.562±0.374)、骨小梁面积百分比(26.531±3.388)%均明显增加(Ρ<0.05),而骨小梁分离度明显降低(Ρ<0.05)。灌胃后9周,大鼠股骨的弹性载荷、刚性系数等生物力学参数均明显增加,骨小梁厚度、骨小梁数量等形态学参数也均出现明显变化,差异有统计学意义(Ρ<0.05)。结论骨化三醇能够改善糖尿病大鼠股骨显微结构的退化及生物力学性能的降低,增加力学结构的稳定性,治疗骨质疏松。
Objective To investigate the effect of calcitriol on the mechanical structure of femur in diabetic rats. Methods Diabetic rats were induced by streptozotocin (STZ) and were randomly divided into diabetic group (DM) and calcitriol-orally administered group (VD), healthy rats of same age as normal control group (NC). Calcitriol after 6 weeks, 9 weeks, take the right femoral biomechanical testing; take the left femur to prepare decalcified bone slices, bone morphological observation and measurement. Results Compared with the NC group, the biomechanical parameters of the femur in each DM group were significantly decreased (P <0.05). The histomorphometric parameters showed that the trabecular number, trabecular thickness and trabecular area percentage were significantly (P <0.05), while the trabecular separation was significantly increased (P <0.05). Biomechanical parameters of rat femur at 6 weeks after intragastric administration of calcitriol showed that the maximum load (132.47 ± 11.00) and fracture load (115.37 ± 7.64) were significantly increased (P <0.05) compared with the DM group at the same period The parameters of trabecular (3.562 ± 0.374) and trabecular area (26.531 ± 3.388)% were significantly increased (P <0.05), while the trabecular separation was significantly lower (P <0.05). At 9 weeks after gavage, the biomechanical parameters of rat femur, such as elastic load and stiffness coefficient, increased significantly, and the morphological parameters such as trabecular thickness and trabecular number also showed significant changes (P < 0.05). Conclusion Calcitriol can improve the degeneration and biomechanical properties of diabetic femur, increase the stability of mechanical structure and treat osteoporosis.