目的:从抗氧化应激角度比较碘与海藻玉壶汤对碘缺乏甲状腺肿的干预机制。方法:选4周龄Wist-ar大鼠,制成碘缺乏动物甲状腺肿模型。随机分成4组:除正常对照组(NC)饲正常饲料外,其余组饲以低碘饲料。NC组和模型对照组(MC)每日灌服等体积双蒸水。单纯高碘组(PHI)灌服含碘1900μg/L的高碘水,海藻玉壶汤(HZ)每日灌服按与人相同体表面积折算的海藻玉壶汤。在给予处理因素3周和11周后处死大鼠。采用生化方法测定大鼠血清内谷胱甘肽过氧化酶(GSH-Px)、黄嘌呤氧化酶(XOD)和超氧化物歧化酶(SOD)的活力,以及丙二醛(MDA)及过氧化氢(H2O2)的含量。采用免疫组化方法检测甲状腺中4-羟基壬烯醛(4-HNE)的表达。采用Western印迹及免疫组化技术对过氧化物氧化还原酶5(PRDX5)在大鼠甲状腺的表达及分布进行了分析。结果:用药11周后,HZ组SOD活力呈现上升趋势,明显高于NC组和PHI组(P<0.05或P<0.001)。HZ组11周较3周时GSH-PX活性有所增加。11周时PHI组H2O2含量明显高于NC组,并且MDA含量明显高于其他组(均P<0.05)。11周时XOD活性PHI组和HZ组均明显高于NC组和MC组(P<0.001或P<0.01)。甲状腺4-HEN表达PHI组明显高于MC组(P<0.05),HZ组明显低于NC组和PHI组(P<0.05)。甲状腺PRDX5表达免疫组化和Western blot结果均显示HZ组较NC和PHI组显著增高(P<0.05)。结论:海藻玉壶汤通过抗氧化应激,可使甲状腺肿恢复更完全,并未造成甲状腺细胞的损伤。 Objective: To compare the intervention mechanism of iodine and seaweed Yuhu soup on iodine deficiency goiter from the perspective of antioxidant stress. Methods: 4-week-old Wist-ar rats were selected to make goat-deficient animal model of goiter. Randomly divided into 4 groups: except normal control group (NC) fed normal feed, the other group fed low iodine feed. The NC group and the model control group (MC) were orally dosed with equal volumes of double distilled water daily. Pure iodine group (PHI) fed with iodine 1900μg / L of iodine, seaweed juguhuan (HZ) daily fed with the same body surface area conversion of seaweed jade pot soup. Rats were sacrificed 3 and 11 weeks after the treatment factors were given. Biochemical methods were used to determine the activities of glutathione peroxidase (GSH-Px), xanthine oxidase (XOD) and superoxide dismutase (SOD) in serum of rats, and malondialdehyde (MDA) Hydrogen (H2O2) content. Immunohistochemistry was used to detect the expression of 4-hydroxynonenal (4-HNE) in thyroid. The expression and distribution of peroxiredoxin 5 (PRDX5) in rat thyroid were analyzed by Western blot and immunohistochemistry. Results: After 11 weeks of treatment, the activity of SOD in HZ group showed an upward trend, which was significantly higher than that in NC group and PHI group (P <0.05 or P <0.001). GSH-PX activity increased in HZ group at 11 weeks compared with 3 weeks. At 11 weeks, the content of H2O2 in PHI group was significantly higher than that in NC group, and the content of MDA in PHI group was significantly higher than that in other groups (all P <0.05). At 11 weeks, the activity of XOD in PHI group and HZ group were significantly higher than that in NC group and MC group (P <0.001 or P <0.01). The expression of thyroid 4-HEN in PHI group was significantly higher than that in MC group (P <0.05), while that in HZ group was significantly lower than that in NC group and PHI group (P <0.05). Immunohistochemistry and Western blot results of thyroid PRDX5 expression showed that HZ group was significantly higher than NC and PHI group (P <0.05). Conclusion: Seaweed Yuhu Tang through anti-oxidative stress, can be more complete recovery of goiter, did not cause thyroid cell damage.